BACKGROUND Neuraminidase (NA) is one of the key surface protein of the influenza virus, and has been established as a primary drug target for anti-influenza therapies. This study aimed to screen bioactive herbal extracts from some medicinal plants traditionally used in Lingnan Chinese Medicines by NA activity high-throughput screening assay. METHODS One hundred ninety herbal extracts from 95 ...

NADP(+)-isocitrate dehydrogenase (NADP(+)-IDH) from the dinitrogen-fixing filamentous cyanobacterium Anabaena sp. strain PCC 7120 was purified to homogeneity. The native enzyme is composed of two identical subunits (M(r), 57,000) and cross-reacts with antibodies obtained against the previously purified NADP(+)-IDH from the unicellular cyanobacterium Synechocystis sp. strain PCC 6803. Anabaena N...

In this study the fresh water grown Blue-Green Algae (Anabaena ambigua) is studied for its maximum growth. This Blue-Green Algae (Anabaena ambigua) growth is studied in detail in three different media viz.,Fog’s media,BG-11, BBM , there was a significant change observed in growth of the species Anabaena ambigua at different light intensities and different media, during 15-days growth. Growth wa...

The construction of an integrative shuttle expression vector and potential utility was reported in Escherichia coli and Anabaena (Nostoc) sp. strain PCC 7120. The vector comprised of the following elements: (a) an intergenic non-coding region from Anabaena to facilitate its genomic integration (b) a strong functional PpsbAI promoter from Anabaena for desired gene expression and (c) neomycin pho...

By means of potentiometric titrations, the copper-complcxing properties of organic compounds released by 21 algal species are characterized. Most eucaryotic species relcascd measurablc amounts (lo-” M) of copper-complexing agents that could be modeled as weak organic acids with log{*&.,,,} = 0.5. Of seven blue-green algal species studied, four produced about 5 x lo+ M concentrations of strong c...

Großer Müggelsee and Langer See are limnologically similar but during an investigation from 1990 until 1993 marked differences were observed in the species that dominated during the summer blooms. Two distinct planktonic communities could be recognised, one dominated by ”Planktothrix agardhii” and the other by ”Aphanizomenon flos-aquae/Microcystis spp.”. A sensitive moment for the differentiati...

Heterocysts of free-living cyanobacteria lack ribulose-1,5-bisphosphate carboxylase activity. Nevertheless, using in situ hybridizations, we demonstrate that transcripts for the rbcL and rbcS genes are present in both heterocysts and vegetative cells of Anabaena spp. in association with, or isolated from, the Azolla-Anabaena symbiosis. In contrast, rbcLS transcripts were detected only in vegeta...

The nifV and leuA genes, which encode homocitrate synthase and alpha-isopropylmalate synthase, respectively, were cloned from the filamentous cyanobacterium Anabaena sp. strain PCC 7120 by a PCR-based strategy. Since the N-terminal parts of NifV and LeuA from other bacteria are highly similar to each other, a single pair of PCR primers was used to amplify internal fragments of both Anabaena str...

Methylation by Ava methylases in Escherichia coli increases the efficiency to transfer of Tn5 in pBR322bla:: Tn5 from E. coli to Anabaena sp. strain PCC 7120 by conjugation. Following conjugation, Tn5 but not pBR322 sequences were found at many different positions in the Anabaena chromosome. This procedure was used to mutagenize, tag, and clone a previously unrecognized gene required for nitrog...