Abstract Background Blood biomarkers of Alzheimer’s disease (AD) attract much attention researchers recently. In clinical studies, repeated freeze/thaw cycles often occur and may influence the stability biomarkers. This study is to investigate amyloid‐β 1‐40 (Aβ ), 1‐42 ) total Tau protein (T‐Tau) in plasma over cycles. Method Plasma samples from healthy control (HC, n = 3), AD patients (AD, 3)...