نتایج جستجو برای: î3 h2ax

تعداد نتایج: 2028  

2018
Zhen Yang Qing-De Wa Chao Lu Wei Pan Zi-Mo Lu Jun Ao

Osteosarcoma is a kind of high-risk sarcoma of the skeleton typically observed in people under 25 years old. Currently, radiotherapy is widely applied in cancer treatment. However, osteosarcoma is radioresistant and accordingly new, more effective radiosensitizers are needed. miRNAs have been reported to play an important role in osteosarcoma radiosensitivity. We examined the modulating effect ...

Journal: :EMBO reports 2009
Olivier Sordet Christophe E Redon Josée Guirouilh-Barbat Susan Smith Stéphanie Solier Céline Douarre Chiara Conti Asako J Nakamura Benu B Das Estelle Nicolas Kurt W Kohn William M Bonner Yves Pommier

Ataxia telangiectasia mutated (ATM), the deficiency of which causes a severe neurodegenerative disease, is a crucial mediator for the DNA damage response (DDR). As neurons have high rates of transcription that require topoisomerase I (TOP1), we investigated whether TOP1 cleavage complexes (TOP1cc)-which are potent transcription-blocking lesions-also produce transcription-dependent DNA double-st...

2012
Aida Muslimovic Pegah Johansson Ulla Ruetschi Ola Hammarsten

Agents that induce DNA double-strand breaks (DSBs), such as ionizing radiation, are frequently used in cancer therapy. H2AX is rapidly phosphorylated in response to DSBs and serves as a way to measure the extent of DSBs induced in patient cells. We have previously reported a flow cytometry-based method for measuring H2AX phosphorylation in patient cells undergoing radiotherapy. To be able to im...

Journal: :The Journal of biological chemistry 1998
E P Rogakou D R Pilch A H Orr V S Ivanova W M Bonner

When mammalian cell cultures or mice are exposed to ionizing radiation in survivable or lethal amounts, novel mass components are found in the histone H2A region of two-dimensional gels. Collectively referred to as gamma, these components are formed in vivo by several procedures that introduce double-stranded breaks into DNA. gamma-Components, which appeared to be the only major novel component...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2005
Markus Löbrich Nicole Rief Martin Kühne Martina Heckmann Jochen Fleckenstein Christian Rübe Michael Uder

Ionizing radiation can lead to a variety of deleterious effects in humans, most importantly to the induction of cancer. DNA double-strand breaks (DSBs) are among the most significant genetic lesions introduced by ionizing radiation that can initiate carcinogenesis. We have enumerated gamma-H2AX foci as a measure for DSBs in lymphocytes from individuals undergoing computed tomography examination...

2014
Julia A. Yaglom Christopher McFarland Leonid Mirny Michael Y. Sherman

DNA instability is an important contributor to cancer development. Previously, defects in the chromosome segregation and excessive DNA double strand breaks due to the replication or oxidative stresses were implicated in DNA instability in cancer. Here, we demonstrate that DNA instability can directly result from the oncogene-induced senescence signaling. Expression of the activated form of Her2...

2011
Yemin Wang Jen-Wei Huang Ming Li Webster K. Cavenee Patrick S. Mitchell Xiaofeng Zhou Muneesh Tewari Frank B. Furnari Toshiyasu Taniguchi

Precise regulation of DNA damage response is crucial for cellular survival after DNA damage, and its abrogation often results in genomic instability in cancer. Phosphorylated histone H2AX (gH2AX) forms nuclear foci at sites of DNA damage and facilitates DNA damage response and repair. MicroRNAs (miRNA) are short, nonproteinencoding RNA molecules, which posttranscriptionally regulate gene expres...

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