BACKGROUND Current methods of ethanol production from lignocelluloses generate a mixture of sugars, primarily glucose and xylose; the fermentation cells are always exposed to stresses like high temperature and low nutritional conditions that affect their growth and productivity. Stress-tolerant strains capable of using both glucose and xylose to produce ethanol with high yield are highly desira...

Part I issued January 1970 PAGE Immunofluorescence Examination of Typical Staphylococcus aureus Variants and L-forms for Clumping Factor. By K. L. ELKINS, R. M. HYDE and F. C. KELLY . Basidiospore Germination in the Wood-destroying Fungus Lenzites saepiaria. By H. W. SCHELD and J. J. PERRY Some Properties of the Galactanase Secreted by Phytophthora infestnns (Mont.) De Bary. By M. KNEE and J. F...

Lactosucrose (4(G)-beta-D-galactosylsucrose) is an oligosaccharide consisting of galactose, glucose, and fructose. In this study, we prepared lactosucrose from lactose and sucrose using a levansucrase derived from Zymomonas mobilis. Optimum conditions for lactosucrose formation were 23 degrees C, pH 7.0, 18.0% (w/v) lactose monohydrate, and 18% (w/v) sucrose as substrates, and 1 unit of enzyme/...

Zymomonas mobilis produces two extracellular sucrases namely levansucrase (sac B) and sucrase (sac C). A mutant strain of Z. mobilis CT2 defective in sucrase sac C, constructed earlier by gene disruption, produced higher levels of levan (27.2 g L) than the parent strain B14023 (15.4 g L) from 200 g L of sucrose at 25°C and pH 5.0. Increasing fermentation temperature from 25 to 35°C enhanced eth...

Zymomonas mobilis is a bacterium that can produce ethanol by fermentation. Due to its unique metabolism and efficient ethanol production, Z. mobilis has attracted special interest for biofuel energy applications; an important area of study is the regulation of those specific metabolic pathways. Small RNAs (sRNAs) have been studied as molecules that function as transcriptional regulators in resp...

Pyruvate decarboxylase (PDC, EC 4.1.1.1) is a thiamin diphosphate-dependent enzyme about which there is a large body of structural and functional information. The active site contains several absolutely conserved ionizable groups and all of these appear to be important, as judged by the fact that mutation diminishes or abolishes catalytic activity. Previously we have shown [Schenk, G., Leeper, ...

background and objective: sorbitol is a non-toxic and slightly hygroscopic compound with different applications. zymomonas mobiles produces sorbitol from sucrose or mixtures of glucose and fructose (formation is coupled with the dehydrogenation of glucose to glucono-δ- lactone). recombinant zymomonas mobilis may produce sorbitol and gluconic acid from glucose and fructose using different divale...