In this study, we produced a polyclonal antibody against unprocessed chicken myostatin and examined the effect of in ovo administration of the antibody on posthatch chicken growth and muscle mass. A PCR-amplified unprocessed chicken myostatin cDNA was cloned into an Escherichia coli expression vector, and myostatin proteins were expressed. Recombinant myostatin purified by electro-elution of th...

Two antigens were purified from culture filtrates of Listeria monocytogenes 7973 by the following procedure: (i) acid precipitation with 4 n HCl at pH 3.7, (ii) Sephadex G-75 column fractionation, (iii) diethylaminoethyl-Sephadex A50 batchwise adsorption, and (iv) rechromatography on Sephadex G-75. This procedure resulted in the resolution of two distinct antigens. One antigen, designated a hem...

A single lipophosphoprotein complex, vitellogenin, was isolated and purified from the plasma of oestrogen-stimulated female toads by preparative ultracentrifugation and chromatography on TEAE-cellulose (triethylaminoethylcellulose). The protein contains 12% lipid, 1.5% phosphorus, 1.6% calcium and smaller amounts of carbohydrates and biliverdin. In amino acid composition it is identical with to...

an experiment was conducted to evaluate the effect of fennel essence on performance, egg quality and ovarian morphology of commercial laying eggs for ten weeks. in this experiment, one hundred and forty 30-wk-old commercial egg laying type hy-line (w36) were randomly assigned into 4 treatments with 5 replicates and 8 birds per each. treatments were a control (0.0% essence) and diets containing ...

The present study was conducted to investigate the variation in the lipid profile between Egyptian breed Fayoumi and commercial strain ISA-Brown. Forty 32-wk-old ISA-Brown and thirty-five 36-wk-old Fayoumi were housed individually in separated layer cages in opensystem housing. Four weeks later, Body weight was recorded and egg weight, number, and composition were measured. In addition, lipid p...

This present study was conducted to evaluate the effects of dietary inclusion of 4, 8 and 12 g kg-1 phytogenic feed additives mixture on performance, egg quality, ovary parameters, serum biochemical parameters and yolk trimethylamine level in laying hens. The results of experiment have shown that egg weight was increased by supplementation of 12 g kg-1 feed additive wherea...

Riboflavin carrier (or binding) protein (RCP) was isolated from the Emu egg yolk (Dromaius novaehollandia) for the first time. In the present study an attempt has been made to isolate and purify the RCP in two steps, DEAE Sephadex A-50 ion exchange chromatography and the final purification was achieved on sephadex G-100.The purity of the protein was judged on cylindrical and slab gel electropho...

Fish arsik soup is a popular traditional food in North Sumatera especially Batak ethnic. This research aimed to evaluate the effects of different dose mackerel fish on body weight and LDL blood level rats that were given cholesterol rich feed namely quail egg yolk. Research was performed by using Randomized Group Design. Forty male grouped into 5 groups. The first group (K1) negative control wh...

Chlamydia trachomatis grown in the yolk sac of embryonated eggs was purified by centrifugation on continuous isopycnic Renografin density gradients. A band of chlamydial particles with a buoyant density of 1.20 contained 70% of the starting particles, and electron microscopy revealed the virtual absence of contaminating egg material. Centrifugation on Renografin gradients caused only a moderate...

We report a rare case of a retroperitoneal germ cell tumor in a 31 years old male who presented with an acute abdomen. He gave a six-months history of back pain and significant weight loss. Clinical examination and ultrasound of the testes didn't show any primary focus. Intraoperative findings showed a retrolperitoneal midline mass that has ruptured into the peritoneal cavity and has invaded in...