The objective of this study was to evaluate the effect cryoconcentration assisted by centrifugation-filtration on bioactive compounds and microbiological quality aqueous maqui (Aristotelia chilensis (Mol.) Stuntz) calafate (Berberis microphylla G. Forst) extracts pretreated with high-pressure homogenization (HPH). Aqueous were prepared from fresh fruits which treated HPH (predefined pressure nu...

The herpes simplex virus type 1 (HSV-1) (strain 17) DNA polymerase gene has been cloned into an Escherichia coli-yeast shuttle vector fused to the galactokinase gene (GAL-1) promoter. Genes controlled by the GAL-1 promoter are induced by galactose, uninduced by raffinose, and repressed by glucose. Cell extracts from a strain of Saccharomyces cerevisiae harboring this vector (Y-MH202, expresser ...

The antibacterial effect of aqueous extracts at a concentration of 1% (v/v) and 5% (w/w) of oregano, marjoram, sage and licorice herbs, widely used as soft drinks and food flavors, were evaluated against E. coli and B. subtilis under in vitro conditions in tryptone soya yeast extract broth at 35°C and in milk and labneh respectively. All tested herbal aqueous extracts demonstrated an inhibitory...

Enzymatic activation of methionine has been clearly established as a prerequisite for the transfer of the methyl group of this compound to a variety of methyl acceptors (1). The reaction involves an ATP’-dependent conversion of methionine to AMe. Enzyme systems capable of catalyzing this activation have been found in the tissues of many mammalian species (2, 3) and in extracts of Escherz&a coli...

Commiphora molmol oleoresin (Family Burseraceae) is traditionally used for treatment of many ailments such as ulcers, abscesses and wounds. The aim of this study was to evaluate the antimicrobial and antioxidant activities of C. molmol extracts in vitro (essential oils and ethanol extract). The antimicrobial activities of extracts were evaluated by disc diffusion and micro-broth dilution assays...

Pyruvate decarboxylase has been shown to be more heat labile in extracts of a mutant strain than in extracts of the parent yeast strain. Mutant extracts were found to contain higher levels of proteolytic enzymes than wild-type extracts, although aging of the latter preparations resulted in the activation of inactive pmteases. A partially purified protease preparation was shown to degrade pyruva...

Analysis of cells of Lactobacillus plantarum, starved or undergoing induction, showed no 3', 5'-cyclic adenosine monophosphate (cAMP). Neither adenyl cyclase nor 3', 5'-cAMP phosphodiesterase was detected in extracts. Extracts of L. plantarum did not inhibit these two enzymes of Escherichia coli K-12, strain W1435. Incubation of adenosine triphosphate (ATP)-U-(14)C with cells or various cell-fr...