background: glutathione (gsh) is a non-protein thiol compound, which plays an important role in the response to oxidative stress and nutritional stress. the aim of this study was to isolate indigenous s. cerevisiae strains capable of effectively produce gsh. methods: one hundred-twenty sweet fruit samples were collected. the strains were isolated on yeast glucose chloramphenicol (ygc) agar medi...

Background: Human alpha 1-antitrypsin (AAT) is a monomeric glycosylated protein; it is the potent inhibitor of a whole range of serine proteases and protects tissues against their destructive effects. The human plasma-derived AAT, which is currently used to augment the AAT level in patients, is limited due to high cost and source limitation. Recombinant production of AAT can be considered as a ...

The production of auxin by yeast ceils resembles the formation observed in other organisms such as Rhizopus and Rkizobiura (Thimann, 1939) I which "release" auxins into their culture medium. Thus, we have found far more auxin in the growth medium of yeast than KSgl and Kostermans 2 extracted from the cells. The amounts of auxin in the growth medium were found to vary considerably with the age o...

Here, we describe instructions for the assembly of an array of miniature (20-mL) chemostats or "ministats" built from relatively inexpensive off-the-shelf parts. In experiments with yeast cultures, we have observed reproducibility in cellular physiology, gene expression patterns, and evolutionary outcomes with different ministats as well as between ministats and commercial large-volume platform...

SYNOPSIS The tetrazolium medium described is easily prepared and serves as a rapid, relatively accurate and simple means of differentiating Candida species, particularly C. albicans, from other yeasts. Culture on this medium does not replace other techniques used for yeast identification. No one test alone will suffice and final identification must rest on an overall pattern of characteristics....

We examined the morphological changes in mitochondria, mitochondrial nucleoids (mt-nucleoids), and actin cytoskeleton yeast Saccharomyces cerevisiae during transition from anaerobic to aerobic culture by immunofluorescence microscopy, DAPI staining, rhodamine-phalloidin staining. The cells grown anaerobically stationary phase contained a small number of 7.3 per cell on average. When were transf...

Gas-liquid chromatographic retention times for 20 purines or purine nucleosides, 14 of which are highly active cytokinins, are reported. With one exception, all of the naturally occurring cytokinins are separated.Ethyl acetate extraction of yeast transfer RNA hydrolysates and of culture filtrates of Agrobacterium tumefaciens gave sufficient concentration of the naturally occurring cytokinins fo...

Weare reporting an efficient method for microbial production of 4-thiouridine as an alternative to chemical synthesis. A loopful of a 10-day slant culture of S. libani NK-1 10134 grown on yeast extractstarch agar was inoculated into a 500-ml Erlenmeyer flask containing 1 10 ml ofa seed mediumcomposed of soluble starch 2.0%, glucose 0.5%, peptone 0.5%, yeast extract 0.5%, soybeanmeal0.5%, K2HPO4...