Phytohemagglutinin (PHA) is a lectin, obtained from the red kidney bean that binds to the membranes of T-cells and stimulates metabolic activity, cell division, etc. The object of this research was the comparison between self made PHA (Indigenous) and imported commercial one, following conventional and High Resolution Cell Synchronization technique (HRCS) .From each blood sample of healthy indi...

the aberrant assembly of proteins into fibrillar aggregates is accused to be the primary cause of pathogenesis of neurodegenerative diseases. but the structural determinants of protein fibrils that are responsible for cell dysfunction are not yet clear. in the current study, cell culture, spectroscopic techniques as well as theoretical and structural investigations were used to determine the ab...

Suspension cultured soybean (Glycine max [L.] Merr.) cells of four cultivars (Wilis, Lumut, Kalmit, Doko RC) were compared for their response to different fungal and bacterial elicitors. Cells were treated either with crude cell wall extracts of the fungal pathogens Phytophthora sojae (Pmg-elicitor) and Rhizoctonia solani (Riso-elicitor) or with two isolates of the bacterial pathogen Pseudomona...

background ocular adenoviral infections occur throughout the world in both sporadic and epidemic forms. in the present study we determined the frequency of adenoviral conjunctivitis in two referral university hospitals in tehran by cell culture and pcr method. materials and methods specimens were scraped from the lower palpebral conjunctiva of 115 patients with conjunctivitis who had referred t...

Phytohemagglutinin (PHA) is a lectin, obtained from the red kidney bean that binds to the membranes of T-cells and stimulates metabolic activity, cell division, etc. The object of this research was the comparison between self made PHA (Indigenous) and imported commercial one, following conventional and High Resolution Cell Synchronization technique (HRCS) .From each blood sample of healthy indi...

background: trigonella foenum-graecum l. commonly known as fenugreek is a rich source of important medicinal metabolite, i.e. trigonelline. objective: in this study, hairy roots culture as a novel method for trigonelline production was evaluated. methods: for optimizing the hairy roots culture of trigonella foenum-graecum, three strains of agrobacterium rhyzogenes (atcc15834, msu440 and k599) v...

the endotoxin dose response of recruited and activated tissue macrophages isolated from a rat model of liver injury has been measured after 24 hours in in vitro culture, with respect to their production of a lysosomal enzyme, n-acetyl-glucosaminidase (tlag). there were no significant differences in the nag activity in cell lysates or supernatants following a two-hour exposure of the macrophages...

background and objectives: silymarin is a unique flavonoid complex with documented hepatoprotective properties. silybum  marianum hairy root culture as a source for producing silymarin has been an important strategy for study the cell signaling pathway. in the present investigation trichoderma strains- silybum marianum hairy root cultures interactions have been studied. methods: the effects of ...