In this study, moisture content, free fatty acids, peroxide, iodine value, unsaponifiable matter, saponification value, fatty acid composition and tocopherol contents of wheat germ oil obtained by SC-CO2 extraction and cold press technology were investigated. Moisture, free fatty acid (FFA), peroxide value, iodine value, unsaponifiable matter and saponification value of cold pres...

BACKGROUND Whole plants or plant cell cultures can serve as low cost bioreactors to produce massive amounts of a specific protein for pharmacological or industrial use. To maximize protein expression, translation of mRNA must be optimized. Many plant viral RNAs harbor extremely efficient translation enhancers. However, few of these different translation elements have been compared side-by-side....

It has been demonstrated for the first time that a wheat germ extract prevents colonic cancer in laboratory animals. Four-week-old inbred male F-344 rats were used in the study. Colon carcinogenesis has been induced by azoxymethane (AOM). Ten rats served as untreated controls (group 1). For the treatment of the animals in group 2, AOM was dissolved in physiologic saline and the animals were giv...

Dietary spermidine is emerging as a substance with promising health-promoting and healthy ageing-promoting properties. Its autophagy-inducing potential has been especially well-documented. This process suspected to be at the root of life-prolonging effects fasting in-vivo supplementation. In humans, high dietary intake associated lifespan increase 5.7 years. Numerous studies provide indications...

This study optimised the conditions for ultrasound-assisted extraction (UAE) of defatted wheat germ protein (WGP) and evaluated its effect on functional properties. Single-factor orthogonal experiment designs were combined to optimise UAE condition. The WGP reached highest level, at 88.66%, with a solid : liquid ratio 1 : 25 g·mL–1, pH value 9.0, ultrasonic time 10 min, power 400 W. U...

Guanine, queuine-tRNA transglycosylase has been purified from wheat germ to homogeneity. The specific activity is 2,000 pmol h-1 mg-1 of protein. The enzyme has an apparent Mr = 140,000. It migrates as a single band with Mr = 68,000 on electrophoresis in sodium dodecyl sulfate gels, indicating two Mr = 68,000 subunits. Both guanine (Km = 6.0 X 10(-8) M) and queuine (KI = 9.5 X 10(-8) M) are sub...

It has been reported [Smith, McWilliams & Hass (1986) Biochem. Biophys. Res. Commun. 136, 336-340] that addition of certain metal ions, notably Co2+ and Mn2+, promoted the refolding of denatured phosphoglycerate mutase from wheat germ. We have re-investigated these experiments and have shown that, when precautions are taken to avoid artefacts in the assay system, the metal ions do not promote a...

Rational: Smoking kills more than one billion current smokers worldwide prematurely due to various tobacco-related diseases. Smoking contributes to multiple well-documented adverse health effects, including heart disease, pulmonary disease and lung and other cancers in both industrialized and developing countries. Tobacco smoking is a leading cause of preventable disease and death. If the curre...