The pH-regulated expression of the acid (AXP) and alkaline (AEP) extracellular proteases of the yeast Yarrowia lipolytica 148 was analysed. Expression in batch and continuous cultures was determined at the mRNA level by Northern blotting, and at the enzyme level by enzyme assays and Western blotting. Culture pH regulated AEP and AXP expression predominantly at the level of mRNA content. Highest...

pod borer is one of the main factors for yield decrease of chickpea. therefore, breeding of chickpea for resistance to this pest is important. modified cry1ac gene of bacillus thuringiensis in pcry1ac-nptii plasmid containing twin t-dna for cry1ac and nptii genes have been used for transformation of chickpea. at last 38 plants had nptii gene that 36 plants of them had cry1ac gene and two plants...

background homocysteine is a sulfur-containing amino acid which formed (mainly in the liver) during the metabolism of methionine. prior studies indicated the important role of hyperhomocysteinemia in pathogenesis and progression of alcoholic liver disease, liver steatosis and cirrhosis. one of the most important mechanisms by which homocysteine promote the development of hepatic injury is oxida...