A modified adsorption-elution method for the concentration of seeded rotavirus from water samples was used to determine various factors which affected the virus recovery. An enzyme-linked immunosorbent assay was used to detect the rotavirus antigen after concentration. Of the various eluents compared, 0.05M glycine, pH 11.5 gave the highest rotavirus antigen recovery using negatively charged me...

Membrane filtration has become an accepted technology for the removal of pathogens from drinking water. Viruses, known to contaminate water supplies, are too small to be removed by a size-exclusion mechanism without a large energy penalty. Thus, functionalized electrospun membranes that can adsorb viruses have drawn our interest. We chose a quaternized chitosan derivative (HTCC) which carries a...

Contamination of lakes and ponds plays an essential role as a reservoir of avian influenza A virus (AIV) in the environment. A method to concentrate waterborne AIV is a prerequisite for the detection of virus present at low levels in water. The aim of this study was to develop and validate a method for the concentration and detection of infectious AIV from large volumes of surface water samples...

CARLUCCI, A. F. (Rutgers, the State University, New Brunswick, N. J.), P. V. SCARPINO, AND DAVID PRAMER. Evaluation of factors affecting survival of Escherichia coli in sea water. V. Studies with heatand filter-sterilized sea water. Appl. Microbiol. 9:400404. 1961.-The bactericidal action of sea water was measured as the difference in survival of cells of Escherichia coli in untreated and autoc...

Laboratory column studies were conducted at the Utah Water Research Laboratory, Logan, Utah, to evaluate reovirus removal from drinking water supplies by slow-rate sand filtration (SSF). Columns, constructed to simulate a full-scale SSF field operation, were inoculated with reovirus at ca. 1,000-times-greater concentrations than those typically found in domestic sewage. Reovirus removal and ina...

The importance of a renal factor in the pathogenesis of peripheral cardiac edema has been emphasized in recent publications ( 1-11 ). The work of Futcher and Schroeder (1) indicated that the renal excretion of salt and water was impaired during cardiac decompensation. Numerous other workers (4-6, 10, 11) reported a reduction in renal blood flow, glomerular filtration rate, and sodium excretion ...

Detection of pathogenic Naegleria fowleri in environmental water samples, which is necessary for the prevention of primary amoebic meningoencephalitis, generally requires concentrating the samples. Two concentration techniques, filtration and centrifugation, were used to study the recovery of N. fowleri, in vegetative or cystic form, that had been mixed with the two other thermotolerant Naegler...