this study aimed to investigate the contribution of four common dfnb (“dfn” for deafness and “b” for autosomal resessive locus) loci and gjb2 gene mutations (exon 2) in hearing impairment in individuals living in markazi and qom provinces of iran. forty consanguineous iranian families with at least three affected individuals in family or pedigree who suffer from an autosomal recessive non-syndr...

background: blastocystis is one of the most common parasites, reported from both human and animals. this parasite is more prevalent in regions with low levels of hygiene, close contact with animal and unsuitable disposal systems. the aim of the study was to subtype blastocystis sp., isolated from diarrheic and non-diarrheic patients using sequencing of 18s ribosomal dna. methods: totally, 300 s...

background viral meningitis is an inflammation of the leptomeninges as a manifestation of central nervous system (cns) infection. more than 85% of viral meningitis cases are caused by non-polio enteroviruses. this is the first study on the description of the enteroviruses and the related serotypes involved in viral meningitis in iran. objectives this project was conducted to improve our knowled...

AIMS To develop a polymerase chain reaction (PCR) based technique that would permit the rapid and highly specific detection of hepatitis C virus (HCV) RNA extracted from frozen liver tissue. METHODS Samples of liver tissue from 18 patients undergoing orthotopic liver transplantation were studied. Nine patients were HCV positive. Total RNA was extracted from between one and 10 sections, 10 mic...

A field survey was carried out in districts of southern Karnataka to assess the pospiviroid infections on tomato crop. The plants showing disease symptoms such as severe leaf curling, epinasty, chlorosis, purpling and stunted growth that are characteristics viroid infection were collected along with asymptomatic analysed by RT-PCR. sample from Mandya district showed an amplicon size ~ 361 bp wh...

Newcastle disease is one of the main concerns of poultry farmers. Detection of virulent strains of Newcastle disease virus (NDV) has a great impact on control measures against the disease. In this study RT-PCR was optimized in high sensitivity in order to differentiate the virulent from non-virulent NDV isolates directly in tissue homogenates. The vaccinal NDV strain and known field isolates we...

Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degre...