One of the most effective ways to propagate Lilium is by using tissue culture techniques, preferably through bulblet production. In addition to the surviving percentage of Lilium bulbs after transplantation, the small size of in vitro bulblets compared to commercial bulbs and bulblets‟ dormancy are the most important constraints to commercializing Lilium micropropagation. Different concentratio...

Plants are natural sources of bioactive compounds, and the intensive use wild plants to obtain them, in particular secondary metabolites, depletes biocenoses. Instead, modern biotechnological methods, especially cell tissue culture vitro, make it possible get environmentally friendly, highly productive plant raw materials that able synthesize accumulate specialized substances, which valuable fo...

Background Fertility preservation is an important part of scientific study in the field of reproductive medicine. Ovarian cryopreservation and in vitro follicles culture provide option for fertility conservation in older women or cancer patients. Basic fibroblast growth factor (bFGF) or FGF-2, is member of fibroblast growth factors family which play critical roles in cell migration proliferatio...

Different in vitro culture techniques have been used for rapid plant propagation. Pathogenfree plants have been produced using techniques such as seed culture, meristem culture, micropropagation using axillary or adventitious shoot buds, and somatic embryogenesis. This Bulletin discusses the importance of in vitro culture techniques for the production of pathogen-free medicinal plants such as A...

Plantlets grown in conventional tissue culture systems usually encounter physiological and anatomical abnormalities including inability to photosynthesize, low chlorophyll content, open stomata, lack of a cuticle layer in the leaf, abnormal xylem parenchyma etc. Photoautotrophic and photomixotrophic systems could diminish these problems. The purpose of this study was to increase the chlorophyll...

Background Cryopreservation of ovarian cortex is increasingly used to preserve fertility before cancer therapy. Recently, studies show that Brain-derived neurotrophic factor (BDNF) may be involved in oocyte maturation. Brain-derived neurotrophic factor (BDNF) is member of neurotrophin family that has anti-apoptotic effects on nervous system. Recent researches show that it also plays key role in...

Background: Human umbilical cord blood (HUCB) is an acceptable and readily accessible source of stem cells. There is an ongoing interest in cord blood stem cell therapies; however, little is known about the possible unfavorable effects of laboratory modifications on the isolated HUCB cells. The involvement of miRNAs in several biological processes has been shown. The aim of this study was to ev...

Light is important for the production of metabolites by plant cell culture. It is therefore necessary to evaluate the light conditions with respect to exposure time. Both enhancement and inhibition have been observed depending on exposure time and species. Callus culture of D. nervosa was established and effect of light and passage on cardiac glycoside production was studied. In vitro cultures ...

one of the most effective ways to propagate lilium is by using tissue culture techniques, preferably through bulblet production. in addition to the surviving percentage of lilium bulbs after transplantation, the small size of in vitro bulblets compared to commercial bulbs and bulblets‟ dormancy are the most important constraints to commercializing lilium micropropagation. different concentratio...