نتایج جستجو برای: vitrification

تعداد نتایج: 2354  

2012
Shinsuke Seki Peter Mazur

Intracellular ice is generally lethal. One way to avoid it is to vitrify cells; that is, to convert cell water to a glass rather than to ice. The belief has been that this requires both the cooling rate and the concentration of glass-inducing solutes be very high. But high solute concentrations can themselves be damaging. However, the findings we report here on the vitrification of mouse oocyte...

2015
Budhan S. Pukazhenthi Jennifer Nagashima Alexander J. Travis Guilherme M. Costa Enrique N. Escobar Luiz R. França David E. Wildt

The ability to spur growth of early stage gametic cells recovered from neonates could lead to significant advances in rescuing the genomes of rare genotypes or endangered species that die unexpectedly. The purpose of this study was to determine, for the first time, the ability of two substantially different cryopreservation approaches, slow freezing versus vitrification, to preserve testicular ...

Journal: :Acta cirurgica brasileira 2014
João Marcos de Meneses e Silva Luiz Gonzaga Porto Pinheiro José Alberto Dias Leite Lígia Helena Ferreira Melo Franciele Osmarini Lunardi Rômulo Cesar Costa Barbosa Filho Cindy Vitalino Mendonça

PURPOSE To compare two rat ovary cryopreservation techniques (vitrification vs. slow freezing) and two postmenopausal stages (early vs. late) with regard to graft take. METHODS Thirty-three Wistar rats were submitted to bilateral oophorectomy. One ovary was submitted to histological analysis while the other was cryopreserved by slow freezing or vitrification. The cryopreserved ovary was thawe...

2017
Fabian B. Wadsworth Michael J. Heap David E. Damby Kai-Uwe Hess Jens Najorka Jérémie Vasseur Dominik Fahrner Donald B. Dingwell

During European prehistory, hilltop enclosures made from polydisperse particle-and-block stone walling were exposed to temperatures sufficient to partially melt the constituent stonework, leading to the preservation of glassy walls called 'vitrified forts'. During vitrification, the granular wall rocks partially melt, sinter viscously and densify, reducing inter-particle porosity. This process ...

Journal: :Rheumatology 2008
S E Elizur R C Chian C A Pineau W Y Son H E G Holzer J Y J Huang Y Gidoni D Levin E Demirtas S L Tan

OBJECTIVE To describe a case series of seven women with SLE and other systemic autoimmune rheumatic diseases (SARDs) who required cyclophosphamide therapy and underwent fertility preservation treatments. METHODS Of the seven patients reported here, five women had SLE with nephritis, the sixth had immune thrombocytopenia purpura (ITP) and the seventh had microscopic polyangiitis (MPA) with ren...

Journal: :international journal of reproductive biomedicine 0

background: spermatozoa cryopreservation is used for the management of infertility and some other medical conditions. the routinely applied cryopreservation technique depends on permeating cryoprotectants, whose toxic effects have raised the attention towards permeating cryoprotectants-free vitrification technique. objective: to compare between the application of slow cryopreservation and vitri...

Barati F Rahimi A, Shahriari A

Background: Cryopreservation of ovary for preserving germ cell line is going to be an important issue. However, the physical and chemical stresses during cryopreservation are the most important factors that may influence the post freezing tissue viability and quality. Vitrification is a new approach to bypass the impacts of both stresses for gametes and embryos and it is also suggested for ovar...

2014
Satish Kumar

The aim of this study was to investigate the effect of different cryoprotectants like glycerol and DMSO individually and in combination of both on morphology of sheep oocyte using vitrification. The vitrification of sheep oocyte was performed using 40% glycerol and 40% DMSO individually and in combination of 20% glycerol + 20% DMSO. It was found that combination of cryoprotectants proved more e...

Journal: :علوم باغبانی 0
سیده مهدیه خرازی سید حسین نعمتی علی تهرانی فر عبدالرضا باقری احمد شریفی

carnation is considered as the world's third most important cut flower. tissue culture techniques offer suitable method for micropropagation of this ornamental plant. however, one of the problems during in vitro culture of carnation is vitrification. ratio of ammonium to nitrate and agar concentrations in the medium affect this phenomenon. therefore, in this study the effect of these factors on...

2013
Miki Maehara Masato Sato Masahito Watanabe Hitomi Matsunari Mami Kokubo Takahiro Kanai Michio Sato Kazuaki Matsumura Suong-Hyu Hyon Munetaka Yokoyama Joji Mochida Hiroshi Nagashima

BACKGROUND There is considerable interest in using cell sheets for the treatment of various lesions as part of regenerative medicine therapy. Cell sheets can be prepared in temperature-responsive culture dishes and applied to injured tissue. For example, cartilage-derived cell sheets are currently under preclinical testing for use in treatment of knee cartilage injuries. The additional use of c...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید