The aim of this study was to evaluate cytotoxicity of direct pulp capping materials such as Dycal, Life, ProRoot MTA, and Super-Bond C&B on L929 fibroblasts. Freshly mixed or set materials were prepared and eluted by incubation with cell culture medium for working time period (fresh) or for 6 hours (set). The cells were exposed to media containing elutes for 24 hours, after which the cell survi...

Traumatic or carious exposure of a vital pulp in an immature permanent tooth presents a significant clinical challenge to maintain proper vitality. Currently available procedures include direct pulp capping, complete pulpotomy and partial pulpotomy. This paper reviews the application of partial pulpotomy in immature permanent teeth and provides prognostic and technique guidance.

Caries results in the demineralization and destruction of enamel dentine, as disease progresses, irreversible pulpitis can occur. Vital pulp therapy (VPT) is directed towards preservation prevention progression inflammation. The outcomes VPT are not always predictable, there often a poor correlation between clinical signs symptoms, events occurring at molecular level. inflamed expresses increas...

Here we report the draft genomic sequences of two clinical isolates of Lactobacillus rhamnosus from infected dental pulps representing the initial stages of infection of pulp tissue. Based on 454 FLX+ pyrosequencing, the two clinical isolates infecting vital pulp had a genome length of 2.9 Mbp with distinct genomic signatures.

OBJECTIVE The purpose of this prospective study was: 1) to follow-up a large number of endodontic treatments performed by a single operator, periodically checked over a 5-year period; and 2) to correlate outcome to a number of clinical variables. STUDY DESIGN This prospective study included all consecutive cases during the selected time period. All cases were followed regularly for a 5-year p...

BACKGROUND Successful isolation of human dental pulp stem cells (hDPSCs) has been documented at least 120h after tooth extraction. Viable hDPSCs have been isolated chiefly from cryopreserved healthy molar teeth and their undigested dental pulp tissue. Isolation of hDPSCs from diseased but vital teeth after cryopreservation has not been reported. This study aimed to isolate hDPSCs from cryoprese...

The aim of the current study was to evaluate the effect of a calcium phosphate material equipped with poly (lactic-co-glycolic acid) microspheres for pulp capping, and to measure the dentin bridge formation, when using various concentrations of transforming growth factor (TGF) b1. Preset samples were made (2 mm diameter; 2 mm height), containing 0 (controls), 20, or 400 ng TGF-b1. These were pl...

Dens invaginatus (DI) is a dental malformation occurring in several morphologic types. Consequently, treatment of teeth affected by DI can get complicated because of the complex root canal anatomy. The aim is to describe how to manage a rare report of a DI with necrotic pulp held within the vital pulp of a maxillary lateral incisor. Oral fistula was found on the buccal mucosa of the apex of the...

Stem cell-based therapy has great potential in treating health conditions including cardiovascular, autoimmune, type I diabetes, neurodegenerative and bone and cartilage diseases also in spinal cord injuries, malformations and cancer. In addition to their potential use to treat systemic diseases, stem cell-based therapy also provides a powerful tool to treat oral and dental diseases such as cra...