Direct immunofluorescence and direct immunoperoxidase staining were equally sensitive and specific for detection of herpes simplex virus antigen in lesion specimens, and each method showed 82% agreement with virus isolation results.

Lumpy skin disease (LSD) is a disease of cattle, primarily in Africa and Madagascar and rarely in the Middle East. It is caused by a capripoxvirus that belongs to the family Poxviridae. The disease is of economic importance in endemic areas. Effective control of LSD requires accurate and rapid laboratory techniques to confirm a tentative clinical diagnosis. Comparative studies on different diag...

Measles virus-infected hamster brain tissue remained viable when frozen slowly in medium containing dimethyl sulfoxide. This procedure was essential for isolation of neurotropic measles virus from tissue specimens stored frozen.

context ebola hemorrhagic fever (ehf) is amongst severe and fatal viral hemorrhagic fevers. in february 2014, a strain of the ebola virus appeared in guinea and then in liberia, sierra leone, and mali. the aim of this study was to review the literature on ehf and discuss its routes of transmission and prevention. evidence acquisition we searched medical databases (pubmed, scopus) from july 1970...

nasopharyngeal swab samples from patients with an acute flu-like illness were evaluated for the presence of respiratory syncytial virus (rsv) from october 1996 to march 1998. the relative frequency and seasonal distribution of rsv was assessed. in addition, the virus correlated with specific clinical signs and symptoms. during the study, 268 samples were collected from children under the age of...

background and aims: a multiplex transcription-polymerase chain reaction (m-pcr) was developed for direct detection and discrimination between canarypox virus (cpv) and other avian poxvirus (apv). materials and methods: three compatible primer sets were designed for m-pcr amplification of different loci fpv126, fpv140, and fpv167 located at highly conserved apv genes. results: results showed th...

Foot and Mouth Disease (FMD) is highly contagious disease among cloven-hoofed animals. FMD virus has structural and non-structural proteins. Vp1 is the most immunogenic structural peptides of FMD virus, applied for major vaccine studies. Objective: Construction of Pet28-VP1 cassette for FMD virus type O/IRN/2010 and expression VP1 peptide as the most immunogenic antigen was the aim of this stud...

Human monkeypox, an infectious disease caused by monkeypox virus (MPXV), is endemic to western and central Africa. A LightCycler quantitative PCR (LC-qPCR) system was developed for the diagnosis of this disease, targeting the A-type inclusion body gene (ATI gene) of MPXV. One naive monkey was infected with MPXV Zr-599 (Congo Basin strain) and one with MPXV Liberia (West African strain). Another...

BACKGROUND Dengue fever is an important arboviral disease. The clinical manifestations vary from a mild non-specific febrile syndrome to severe life-threatening illness. The virus can usually be detected in the blood during the early stages of the disease. Dengue virus has also been found in isolated cases in the cerebrospinal fluid, urine, nasopharyngeal sections and saliva. In this report, we...