HER2-low is a novel breast cancer entity, however the ability of proper discrimination between HER2 negative and low still matter debate. This study investigates whether gene expression determined by ODX can differentiate at IHC. Retrospective cohort analysis on 229 patients with early BC tested after surgery in Humanitas Cancer Center Jan 2020 2023. We gathered well-known clinicopathological d...

PURPOSE Nodal micrometastasis and circulating tumor cells detected by multimarker quantitative real-time reverse transcription-PCR (qRT-PCR) may have prognostic importance in patients with colorectal cancer. EXPERIMENTAL DESIGN Paraffin-embedded sentinel lymph nodes from 67 patients and blood from 34 of these patients were evaluated in a prospective multicenter trial of sentinel lymph node ma...

BACKGROUND The proper management of patients infected with dengue virus requires early detection. Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained too late to be of clinical value. The production of DENV NS1 peaks early during infection and its detection can combine the advantages of both d...

qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection and quantification of RNA targets and is being utilized increasingly in novel clinical diagnostic assays. Quantitative results obtained by this technology are not only more informative than qualitative data, but simplify assay standardization and quality management. qRT-PCR assays are most established for t...

Abstract Background Circular RNAs (circRNAs) have been reported to be important regulators of the development and progression various carcinomas. However, role circRNA_100290 in gastric cancer (GC) is still unclear. This study aimed investigate GC invasion metastasis possible underlying mechanism. Methods The expression cells tissues was examined using quantitative real-time polymerase chain re...

Real-time quantitative reverse transcription PCR (qRT-PCR) is a technique widely used to quantify the transcriptional expression level of candidate genes. qRT-PCR requires the selection of one or several suitable reference genes, whose expression profiles remain stable across conditions, to normalize the qRT-PCR expression profiles of candidate genes. Although several butterfly species (Lepidop...

PURPOSE Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT-PCR) methods and to evaluate protocols for optimal high-throughput data analysis. METHODS Retinal mRNA profiles o...