ON October 16, 1968, a nationwide cooperative study was officially begun to assess the therapeutic efficacy of urokinase in acute pulmonary thromboembolism. The beginning of the "Urokinase-Pulmonary Em-bolism Trial" was the culmination of the efforts of many individuals* during the course of several years. The fact that the study is now in progress is in large part a result of extensive coopera...

The immunoperoxidase technique, using antibodies against human urinary urokinase (M, 55,000), was used for the localiza tion of this enzyme in histological preparations of human colon tumors and normal colon tissue. The localization of tissue (vas cular) activator was also investigated using antibodies against enzyme purified from human malignant melanoma. Both the "indirect method" and the per...

Abstract Cancer invasion and metastasis are influenced by a plethora of biochemical processes involve many mechanisms, among which chemotaxis haptotaxis two the main mechanisms directing migration cancer cells Chaplain Lolas (2005). Evidence has been found that release complex enzymes such as urokinase-type plasminogen activator (uPA), degrade surrounding extracellular matrix (ECM), thereby all...

The inhibition of six serine proteinases by a tumour-associated trypsin inhibitor (TATI) was studied using synthetic peptide substrates. Physiological concentrations of TATI inhibited the amidolytic activities of trypsin, plasmin, urokinase and tissue plasminogen activator (tPA). Chymotrypsin, kallikrein and thrombin were also inhibited, but by much higher concentrations of TATI. The ability of...

We describe the use of abciximab to prevent rethrombosis of the basilar artery after transluminal angioplasty. A 60-year-old patient with vertebral basilar insufficiency and acute occlusion of the basilar artery underwent revascularization with urokinase and angioplasty. Despite the repeated use of urokinase and angioplasty under anticoagulation with heparin, the basilar artery immediately reth...

Background/aim—Central retinal artery occlusion (CRAO) is typically associated with a poor visual outcome. Several favourable reports of local intra-arterial fibrinolysis (LIF), which involves the superselective administration of a thrombolytic agent directly into the ophthalmic artery, have appeared in the recent literature. The aim of this study was to critically appraise these studies in a c...

Plasminogen activator activity was studied in bile and perfusate during extracorporeal pig liver perfusion. Plasminogen activator activity in bile was up to 58 times greater than in perfusate. A high level of plasminogen activator activity in bile was also found after the addition of urokinase to the perfusate. These results suggest that bile is the main route of active excretion of plasminogen...

Plasma fibrinolytic activity and plasma inhibitory activity against urokinase and tissue activator were measured in primigravidae with moderate or severe pre-eclampsia and in gestation-matched primigravidae with uncomplicated pregnancy. The mean levels of fibrinolytic activity and inhibitory activity against urokinase and tissue activator did not differ significantly between the pre-eclampsia a...

Two mouse urokinase-type plasminogen activator receptor (muPAR) cDNAs were isolated: muPAR1 is homologous to the human urokinase-type plasminogen activator receptor while muPAR2 codes for a 199 residue protein sharing the first 133 residues with muPAR1. Mouse genomic DNA sequencing indicates that the two different mRNAs arise by alternative splicing. In situ hybridization showed differential ex...

The occurrence and distribution of components of fibrinolysis pathways were determined using immunohistochemical techniques applied to 10 cases of primary carcinoma of the breast, normal breast tissue obtained from two patients undergoing reductive mammoplasty, and three cases of benign breast tumors. Tumor cells stained for urokinase- and tissue-type plasminogen activators, plasminogen activat...