Studies to evaluate more than 100 compounds as inhibitors of urease activity in soils showed that dihydric phenols and quinones were the most effective organic compounds tested and that silver and mercury salts were the most effective inorganic compounds. When the most potent inhibitors were compared using 50 1'8 of inhibitor per g of soil, their effectiveness decreased in the order: catechol >...

An understanding of the integrated relationships among the principal cellular functions that govern the bioenergetic reactions of an organism is necessary to determine how cells remain viable and optimise their fitness in the environment. Urease is a complex enzyme that catalyzes the hydrolysis of urea to ammonia and carbonic acid. While the induction of urease activity by several microorganism...

Studies on enzyme inhibition remain a crucial area in drug discovery since these studies have led to the discoveries of new lead compounds useful in the treatment of several diseases. In this study, protocatechuic acid (PCA), an active compound from Hibiscus sabdariffa L. has been evaluated for its inhibitory properties against jack bean urease (JBU) as well as its possible toxic effect on huma...

Dental caries results from prolonged plaque acidification that leads to the establishment of a cariogenic microflora and demineralization of the tooth. Urease enzymes of oral bacteria hydrolyze urea to ammonia, which can neutralize plaque acids. To begin to examine the relationship between plaque ureolytic activity and the incidence of dental caries, recombinant, ureolytic strains of Streptococ...

patients and methods: this study included 526 subjects. gallstones and bile samples of the patients were cultured. isolates were identified by different biochemical tests and examined for their β-glucuronidase activity, slime production and urease activity. biostatistical analyses were done to determine the relationship between the biliary bacterial factors and the types of gallstones. role of ...

Urease (urea amidohydrolase; EC 3.5.1.5) catalyzes the hydrolysis of urea to yield ammonia and carbamate. The latter compound spontaneously decomposes to yield another molecule of ammonia and carbonic acid. The urease phenotype is widely distributed across the bacterial kingdom, and the gene clusters encoding this enzyme have been cloned from numerous bacterial species. The complete nucleotide ...

THE possibility of an error in the determination of urea in blood by urease methods was brought to our notice by the fact that figures obtained by the direct estimation of urea in human blood corpuscles were obviously too high. This error appears to have been first reported by Behre [1923], who observed that when the soya bean urease method was applied to whole blood the urea obtained was parti...

Helicobacter pylori is the causative agent of most cases of gastritis. Once acquired, H. pylori establishes chronic persistent infection; it is this long-term infection that, is a subset of patients, leads to gastric or duodenal ulcer, gastric cancer or gastric MALT lymphoma. All fresh isolates of H. pylori express significant urease activity, which is essential to survival and pathogenesis of ...

The stomach serves as a barrier to enteric infection because of the antibacterial effect of the hydrochloric acid in gastric juice. In this study, we tested the ability of the enteric pathogen Yersinia enterocolitica to tolerate a pH range of 2.0 to 6.0 and found that under the conditions of a normal human fasting stomach (pH < 3 and a gastric emptying time of 2 h), Y. enterocolitica is highly ...

The study was composed of two parts. A one-factor design was adopted to investigate DMD, CPM, NDFD and ADFD of diet by total feces collection method in trial one. Twelve lambs were allotted randomly to 4 groups of 3 animals (replicates), which were fed with basal diet, basic diet plus 40 mg/kg urease inhibitor, urea diet, urea diet plus 40 mg/kg urease inhibitor. Experimental period consisted o...