Studies to evaluate more than 100 compounds as inhibitors of urease activity in soils showed that dihydric phenols and quinones were the most effective organic compounds tested and that silver and mercury salts were the most effective inorganic compounds. When the most potent inhibitors were compared using 50 1'8 of inhibitor per g of soil, their effectiveness decreased in the order: catechol >...

An understanding of the integrated relationships among the principal cellular functions that govern the bioenergetic reactions of an organism is necessary to determine how cells remain viable and optimise their fitness in the environment. Urease is a complex enzyme that catalyzes the hydrolysis of urea to ammonia and carbonic acid. While the induction of urease activity by several microorganism...

The stomach serves as a barrier to enteric infection because of the antibacterial effect of the hydrochloric acid in gastric juice. In this study, we tested the ability of the enteric pathogen Yersinia enterocolitica to tolerate a pH range of 2.0 to 6.0 and found that under the conditions of a normal human fasting stomach (pH < 3 and a gastric emptying time of 2 h), Y. enterocolitica is highly ...

An electrical transducer for urea is described. The excellent stability of various types of urease enzyme electrodes developed in the author's laboratory is due to understanding the parameters which affect immobilized enzyme stability. Preliminary experiments have shown that a liquid layer of urease trapped in a double cellophane layer over a cation electrode may produce a useful enzyme electro...

Cement is a material which maintains durability of building structure and conservation of cultural heritage. Although hundreds and thousands of successful concrete and buildings are annually constructed worldwide, there are large number of concrete structures (including historical monuments), that deteriorates or become unsafe in loading. Hence ,in order to overcome the short comings of convent...

CONTEXT Multiple diagnostic methods are available for the detection of Helicobacter pylori infection, but at present no single one can be used as the gold standard. OBJECTIVE The aim of this study was to evaluate the diagnostic accuracy of 3 invasive and 2 non-invasive methods for detection of Helicobacter pylori infection in symptomatic children and adolescents. DESIGN Prospective cohort s...

A continuous-flow clinical analyzer for the routine estimation of urea is described that makes use of an immobilized-enzyme nylon-tube reactor as part of a flow-through system (a Technicon AutoAnalyzer I). Results of blood-urea analyses by use of the immobilized urease are compared with determinations made with the diacetyl monoxime method and the urease solution method. Clinical trials carried...

We developed a buffered azide-free urea medium which is sensitive, specific, and nontoxic for rapid detection of Campylobacter pylori in gastric biopsies. Detection of urease produced by the organism provides the basis for the test. The substrate is urea in monobasic sodium phosphate buffer, and phenol red provides indication of the pH change that results from urease activity. A rapid change fr...

Field experiments were conducted during 2019 in two different locations Greece (Athens and Farsala) to evaluate the effect of urea fertilization with inhibitors on agronomic yield quality characteristics cannabis varieties (Cannabis sativa L.), “Uso31”and “Fedora 17”. The experimental design was split-plot four treatments—control, Urea (U), Urease Inhibitor (UI), Nitrification (NI) urease inhib...

OBJECTIVES: Current nonendoscopic tests for Helicobacter pylori include antibody tests and the urea breath test. After the administration of C-urea, serum bicarbonate measurement can identify those infected with H. pylori. In this study, our aims were to determine the accuracy of the urea blood test, and to compare the accuracy of the urea blood test with that of rapid urease testing of gastric...