A simple, rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the simultaneous determination of cilostazol and its pharmacologically active metabolite 3,4-dehydro cilostazol in human plasma using deuterated analogs as internal standards (ISs). Plasma samples were prepared using solid phase extraction and chromatographic...

Ultra Performance Liquid Chromatography (UPLC) was employed to develop a rapid and robust method for the analysis of ziprasidone, both as a drug substance and in the final dosage forms. The application of this method in stability analyses was verified. Tests were carried out according to ICH/FDA guidelines, European Pharmacopeia, and United States Pharmacopeia rules, which take into account fac...

An ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC/Q-TOF-MS) method coupled with a principal component analysis (PCA) was developed and applied toward identifying donkey-hide gelatin, bovine-hide gelatin, pig-hide gelatin, tortoise shell glue, and deerhorn glue. The UPLC-MS data of the trypsin digested samples were subjected to principal component analy...

To identify the compounds absorbed in rat serum after the oral administration of Wu-Jia Sheng-Hua (WJSH) capsule, a traditional Chinese medicine (TCM) compound prescription, an ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC/ESI-MS) method, was established. The chromatographic separation of the absorbed compounds and metabolites was achieved w...

Ephedra sinica polysaccharides have been reported to possess important activities, so quality evaluation of polysaccharides from the genus Ephedra is urgent. In this study, enzymatic digestions were performed to establish multiple saccharide fingerprints by ultra-performance liquid chromatography with electrospray ionization triple quadrupole linear ion trap mass spectrometry (UPLC-ESI-TQ-MS/MS...

A novel, stability-indicating, reversed-phase ultra-performance liquid chromatographic (RP-UPLC) method was developed for the determination of pure drotaverine hydrochloride and ibuprofen in the presence of their impurities and degradation products. The method was developed using a Waters UPLC BEH C18, 100 × 2.1 mm, 1.7 µm column with a flow rate of 0.3 mL/min and detector wavelength at 210 nm....

Abstract We developed and validated an optimized method for quantifying lignans using ultra-performance liquid chromatography (UPLC) performs correlation analysis of growth characteristics contents lignans. The methods determining were by measuring the linearity, limit detection (LOD), quantification (LOQ), accuracy, precision UPLC. All calibration curves showed good linearity ( r 2 ≥ 0.9998) w...

Penyakit tuberkulosis merupakan suatu gangguan pernapasan yang menular dan penyebabnya dari adanya infeksi bakteri Mycobacterium Tuberkulosis (bakteri aerob). Pengobatan tuberculosis biasa digunakan obat lini pertama yakni isoniazid, rifampisin, etambutol pirazinamid. Tujuan penelitian ini adalah untuk meninjau efektivitas pengembangan metode HPLC atau UPLC dengan detektor UV MS konsentrasi dal...

Purpose: To investigate the sensitivity and selectivity of ultra-performance liquid chromatographic (UPLC) quantification bromazepam (BRZ) diazepam (DZP) in pharmaceutical industrial wastewater.
 Methods: Wastewater samples were collected from effluents a plant producing BRZ DZP tablet dosage forms. The was done after their solid-phase extraction. resolution process performed on WatersTM c...

A modified QuEChERS method coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established for the simultaneous determination of eight biogenic amines (BAs) in Xinjiang smoked horsemeat sausages. The conditions UPLC-MS/MS, extraction solvent and purification were optimized. internal standard used quantitative analysis. Results showed that BAs exhi...