GTP cyclohydrolase 1 (GTPCH1) is the rate-limiting enzyme in the de novo synthesis of tetrahydrobiopterin (BH4). GTPCH1 protein degradation has been reported in animal models of several diseases, including diabetes mellitus and hypertension. However, the molecular mechanisms by which GTPCH1 is degraded remain uncharacterized. Here we report a novel non-covalent interaction between polyubiquitin...

Post-translational K63-linked poly-ubiquitination of AKT is required for its membrane recruitment and phosphorylation dependent activation in response to growth-factor stimulation. Current assays for target specific poly-ubiquitination involve cumbersome enzymatic preparations and semi-quantitative readouts. We have engineered a reporter that can quantitatively and in a target specific manner r...

Due to the shortcomings of the traditional drilling way, as well as the complexity and difficult mathematical model of the drilling process, etc, this paper briefly analyses the principle of auto-drilling system on AC frequency conversion and the relationship among all factors that affects on the auto-drilling and puts forward a technology that combines parameters' self-tuning fuzzy control wit...

– World wide drilling is developing for oil, gas or water resources. Electric motors power 75% of the total drilling rigs installation, augmenting to 95% for the case of Romania. Monitoring diesel generators micro-power station and motors of the rig is the second important thing to do and complete the main task of drilling process monitoring. This paper describe a monitoring and control system ...

Drilling samples of lunar soil is an important and indispensable part of China’s lunar exploration project. The drill bit temperature during the drilling process is one of the key concerns for scholars, especially for the hard lunar rock drilling in vacuum. In this article, the simulation analysis of drilling in atmosphere and vacuum is conducted. Moreover, a fiber grating temperature measuring...

Future in-situ lunar/martian resource utilization and characterization, as well as the scientific search for life on Mars, will require access to the subsurface and hence drilling. Drilling on Earth is hard – an art form more than an engineering discipline. The limited mass, energy and manpower in planetary drilling situations makes application of terrestrial drilling techniques problematic. Th...

ANDRILL completed its first season in 2006-07 drilling AND-1B through the McMurdo Ice Shelf (MIS) to a depth of 1,285m below the sea floor, a record for Antarctic margin drilling, with 99% recovery. The alternating glacial-interglacial sediment packages interbedded with volcanics provide a uniquely detailed record of Antarctic glacial and climatic change through the Neogene. This paper summariz...