Interleukin-1beta (IL-1beta) treatment of neonatal rat islets for 24 h induces changes in the expression of 105 of 2,200 proteins, as determined previously by two-dimensional (2D) gel electrophoresis. Nitric oxide (NO) has been implicated as one of the mediators of IL-1beta effects in insulin-containing cell lines and rat islets. The aims of this study were 1) to determine the involvement of NO...

BACKGROUND Resveratrol, a phytochemical present in grapes, berries, and red wines, has been reported to induce apoptosis in various cancer cells. To explore the molecular mechanisms involved in the anticancer activity, the apoptotic activity of resveratrol in hepatic cancer cells was investigated. MATERIALS AND METHODS 1-Dimensional (1D) and 2-dimensional (2D) polyacrylamide gel electrophores...

Abbreviations: PTMs=post-translational modifi cations; PPIs=protein-protein interactions; SDS-PAGE= sodium dodecyl sulfate-polyacrylamide gel electrophoresis; 2D-PAGE=two-dimensional polyacrylamide gel electrophoresis; MS=mass spectrometry; ESI=electrospray ionization; MALDI=Matrix Assisted Laser Desorption Ionization; ESI-MS=electrospray ionization mass spectrometry; MALDI-MS=MALDI mass spectr...

A technique has been developed for the separation of proteins by two-dimensional polyacrylamide gel electrophoresis. Due to its resolution and sensitivity, this technique is a powerful tool for the analysis and detection of proteins from complex biological sources. Proteins are separated according to isoelectric point by isoelectric focusing in the first dimension, and according to molecular we...

Two-dimensional gel electrophoresis (2-DE) is able to separate hundreds to thousands of proteins or polypeptides by coupling IsoElectric Focusing (IEF) in first dimension and Sodium Dodecyl Sulphate PolyAcrylamide-Gel Electrophoresis (SDS-PAGE) in second dimension. This particular configuration is called classical 2-DE: IEF separates proteins in function of their isoelectric point (pI) and SDS-...

UNLABELLED 2D Difference In-Gel Electrophoresis (2D-DIGE) or 2D gel technology is being used as a routine proteomics technique for biomarker discovery. Analyzing such high-dimensional data requires multivariate analysis techniques to be applied. In addition, protein post-translational modification (PTM) information from the 2D gel data is usually overlooked. We report on an R package, digeR, wi...