نتایج جستجو برای: tryptophanase

تعداد نتایج: 360  

Journal: :Cell reports 2014
Lukas Bischoff Otto Berninghausen Roland Beckmann

Elevated levels of the free amino acid L-tryptophan (L-Trp) trigger expression of the tryptophanase tnaCAB operon in E. coli. Activation depends on tryptophan-dependent ribosomal stalling during translation of the upstream TnaC peptide. Here, we present a cryoelectron microscopy (cryo-EM) reconstruction at 3.8 Å resolution of a ribosome stalled by the TnaC peptide. Unexpectedly, we observe two ...

Journal: :Applied and environmental microbiology 1983
T Ujimaru T Kakimoto I Chibata

Conditions for the production of tryptophanase from Achromobacter liquidum and for the conversion of l-serine and indole to l-tryptophan were studied. The enzyme could be produced in amounts as great as 0.750 U/ml (degradation) and 0.294 U/ml (synthesis) by shaking cultures at 30 degrees C in a medium containing dextrin, yeast extract, l-tryptophan, and l-glutamic acid. l-Tryptophan was produce...

Journal: :Journal of clinical microbiology 1993
J N Maslow S M Brecher K S Adams A Durbin S Loring R D Arbeit

Klebsiellae are an important cause of nosocomial infections. The two clinically relevant species, Klebsiella pneumoniae and Klebsiella oxytoca, are differentiated by the ability to produce indole from tryptophan, K. oxytoca being indole positive. We report here the detailed biochemical and molecular analysis of two isolates of Klebsiella, cultured from the same urine specimen, that differed onl...

Journal: :Cell host & microbe 2016
A Sloan Devlin Angela Marcobal Dylan Dodd Stephen Nayfach Natalie Plummer Tim Meyer Katherine S Pollard Justin L Sonnenburg Michael A Fischbach

Renal disease is growing in prevalence and has striking co-morbidities with metabolic and cardiovascular disease. Indoxyl sulfate (IS) is a toxin that accumulates in plasma when kidney function declines and contributes to the progression of chronic kidney disease. IS derives exclusively from the gut microbiota. Bacterial tryptophanases convert tryptophan to indole, which is absorbed and modifie...

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