11-19 ABSTRACT Several different species of annual medics have been tested for the occurrence and concentration of trypsin inhibitors in the seed. Trypsin Inhibitory Activity (TIA) has been in fact related to the level of resistance to biotic and abiotic stresses. Wide variation was found for TIA among species, with the highest values shown by the snail medic (Medicago scutellata). After a simp...

Variants of human secretory trypsin inhibitor were constructed with the aim of producing inhibitors specific for human leukocyte elastase. Models of the hPSTI/HLE and hPSTI/chymotrypsin complexes were generated by computer aided protein design and used to plan better HLE inhibitors. This resulted in the production of the strongest and most specific inhibitors of HLE known.

Specific antisera were prepared against the Bowman-Birk trypsin inhibitor and four other trypsin inhibitors of low molecular weight isolated from soybeans (Glycine max L. cv. Tracy). These antisera were used to detect the presence and amount of the inhibitors in: (a) seeds and protein extracts of soybean meal; (b) seedlings; and (c) the water surrounding the seeds and roots of seedlings. Lectin...

Porcine colostrum and milk were separated into the acid-soluble and casein fractions by acidification followed by centrifuge. The acid-soluble fraction of porcine colostrum was further separated by liquid chromatography and anisotropic membrane filtration. Trypsin and chymotrypsin inhibitory capacity in porcine colostrum, milk and their components was determined by incubating bovine trypsin or ...

Pacific red snapper (Lutjanus peru) is an important commercial species in Mexico with great aquaculture potential; however, digestive physiology still unknown. Therefore, the objective of present work was to characterize proteases L. peru juvenile using biochemical and electrophoretic techniques. Results showed a higher acid protease activity than alkaline proteases, trypsin, chymotrypsin, leuc...

Based on substrate specificity, an alkaline pH optimum, sensitivity to selected proteinase inhibitors, and molecular analysis, we provide evidence for the presence of a trypsin-like serine proteinase in the salivary gland complex (SGC) of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Heteroptera: Miridae). The predominant activity in extracts of the SGC against N(2)-benzoyl-L...

Studies of unfolded and partially folded proteins provide important insight into the initiation and process of protein folding. This review focuses on the use of nmr in characterization of ensembles of proteins that model the early stages of folding. Analysis of an ensemble includes description of the number of conformations, their structure, relative populations, interconversion rates, and dyn...

While steric crowders tend to stabilize globular proteins, it has been found that protein crowders can have an either stabilizing or destabilizing effect, where a destabilization may arise from nonspecific attractive interactions between the test protein and the crowders. Here, we use Monte Carlo replica-exchange methods to explore the equilibrium behavior of the miniprotein trp-cage in the pre...

Chemoenzymatic assay systems are widely used to detect toxicants in various samples, including food and environment specimens. These methods based on the ability of types toxicant specifically inhibit/activate functions individual enzymes or enzyme systems. The present study examines possibility using proteolytic trypsin as a specific marker protease inhibitors different samples. shows that act...

This report is one of a series of papers that introduce and use a new and highly simplified treatment of protein conformations. The first paper (Levitt & Warshel, 1975) outlined fhe approach and showed how it could be used to simulate the “renaturation” of a small protein. The present paper describes fhe representation in some detail and tests the methods extensively under a variety of differen...