new findings of non-disjunction in autosomes of triploid females and of males carrying inversions in their autosomes seem to be desirable. It should be noted that cases of autosomal non-disjunction in males which carry inversions have already been found by Dobzhansky2 (1933) and by J. Schultz (unpublished). Dobzhansky and Darlington have observed cases of non-disjunction of autosomes cytologica...

In the present study, a genome-wide measure of demethylation employing the 5 methylcytidine antibody as well as a gene specific approach by bisulphite sequencing of a house keeping and imprinted genes was investigated to confirm if the active paternal demethylation was occurred correctly in mouse haploid, diploid androgenetic and triploid polyspermic embryos. The results indicated that the acti...

Most banana cultivars are triploid seedless parthenocarpic clones derived from hybridization between Musa acuminata subspecies and sometimes M. balbisiana. M. acuminata subspecies were suggested to differ by a few large chromosomal rearrangements based on chromosome pairing configurations in intersubspecies hybrids. We searched for large chromosomal rearrangements in a seedy M. acuminata ssp. m...

We describe two methods of producing viable and fertile autotetraploid Pacific oyster (Crassostrea gigas Thunberg) based on the use of normal-sized oocytes produced by normal diploid females. Our methods showed that the oocyte size is not a limiting factor for the success of the induction to autotetraploidy. These methods offer means of direct introgression of genetic progress from elite diploi...

Incompatibilities between parental genomes decrease viability of interspecific hybrids; however, deviations from canonical gametogenesis such as genome endoreplication and elimination can rescue hybrid organisms. To evaluate frequency and regularity of genome elimination and endoreplication during gametogenesis in hybrid animals with different ploidy, we examined genome composition in oocytes o...

Maize kernels inheriting the indeterminate gametophyte mutant (ig) on the female side had endosperms that ranged in ploidy level from diploid (2x) to nonaploid (9x). In crosses with diploid males, only kernels of the triploid endosperm class developed normally. Kernels of the tetraploid endosperm class were half-sized but with well-developed embryos that regularly germinated. Kernels of endospe...

Knowing whether triploid hybrids resulting from natural hybridization of parthenogenetic and bisexual species are fertile is crucial for understanding the mechanisms of reticulate evolution in rock lizards. Here, using males of the bisexual diploid rock lizard species Darevskia raddei nairensis and Darevskia valentini and a triploid hybrid male Darevskia unisexualis × Darevskia valentini, we pe...

BACKGROUND Distinction of hydatidiform moles (HMs) from non-molar abortions and sub-classification of HMs are important for clinical practice; yet, diagnosis based solely on morphology is affected by interobserver variability. The objective of this study was to determine the role of DNA flow cytometry in distinguishing molar from non-molar pregnancies. MATERIALS AND METHODS This retrospective...

Genetic analysis of phenotypical traits and marker-trait association in polyploid species is generally considered as a challenge. In the present work, different approaches were combined taking advantage of the particular genetic structures of 2n gametes resulting from second division restitution (SDR) to map a genome region linked to Alternaria brown spot (ABS) resistance in triploid citrus pro...

Genetic investigations with Zea mays have established ten linkage groups. Likewise, cytological investigations have revealed the presence of ten morphologically identifiable chromosomes composing the haploid complement (MCCLINTOCK 1929b). It is the aim of the present investigation to correlate a particular linkage group with a particular chromosome. The method employed has been to obtain 2nfl p...