An important issue in the use of expert systems is the so-called brittleness problem. Expert systems model only a limited part of the world. While the explicit management of uncertainty in expert systems mitigates the brittleness problem, it is still possible for a system to be used, unwittingly, in ways that the system is not prepared to address. Such situation may be detected by the method of...

Idiopathic achalasia is a motility disorder affecting the lower esophageal sphincter. Dysphagia hallmark symptom, but patients may exhibit other symptoms. The aim of this review to compare symptoms globally. PubMed and Google Scholar were filtered from 1952–2021 with search terms achalasia, epidemiology, diet, countries, genetics. A total 14 articles addressed demographics, symptom profiles, ge...

Triple differential cross sections arising from the break up of the H2 molecule by a single photon are presented. The time-dependent close-coupling technique is used to calculate differential cross sections for various geometries. Excellent agreement is found between current work and recent exterior complex-scaling calculations, confirming, for the first time, the absolute magnitude of the trip...

A 14 nt DNA sequence 5'-AGAATGTGGCAAAG-3' from the zinc finger repeat of the human KRAB zinc finger protein gene ZNF91 bearing the intercalator 2-methoxy,6-chloro,9-amino acridine (Acr) attached to the sugar-phosphate backbone in various positions has been shown to form a specific triple helix (triplex) with a 16 bp hairpin (intramolecular) or a two-stranded (intermolecular) duplex having the i...

Attachment of a nondiffusible bromoacetyl electrophile to the 5-position of a thymine at the 5'-end of a pyrimidine oligodeoxyribonucleotide affords sequence-specific alkylation of a guanine base in duplex DNA two base pairs to the 5'-side of a local triple-helical complex. Products resulting from reaction of 5'-ETTTTMeCTTTTMeCMeCTTTMeCTTTT-3' at 37 degrees C with a 29 base pair target duplex a...

Introduction In recent decades, considerable attention has been given to child sexual abuse and many studies have focused on the short-term and long-term consequences of the abuse. Results have continually indicated that sexually abused children and adolescents display a considerable breadth of symptoms, including anxiety, depression, poor self-esteem, substance abuse, selfharm behavior, post-t...

We have characterized triple-helix-mediated inhibition of an artificial bacteriophage promoter with respect to relief of inhibition by incoming RNA polymerases that initiate upstream or downstream from the operator sequence. Whereas oligonucleotide-directed triple-helix formation inhibits the test promoter, promoter activity is restored when the triple-helical complexes are disrupted by transcr...

introduction: the acrosome is a sac shaped organelle contaning lysosomal enzyme that is located on the anterior of sperm head. during the acrosome reaction its content is released in the vicinity of the egg. the acrosome reacted sperm then penetrates through the egg barriers. there are several methods for evaluating the acrosomal status; some of those are complex, expensive and/or non-accurate ...