We are interested in creating artificial gene repressors based on duplex DNA recognition by nucleic acids rather than polypeptides. An in vitro model system involving repression of bacteriophage T7 RNA polymerase initiation has been employed to demonstrate that certain DNA oligonucleotides can repress transcription by site-specific triple-helix formation at two kinds of homopurine operator sequ...

The purpose of the study is to provide empirical evidence about under-researched area university–government relations in building a culture entrepreneurial initiatives inside triple helix model rural region. deploys qualitative case research method based on content analysis project documentation and further internal documents both from universities municipalities. propositions question are guid...

The engagement of a wide range stakeholders has become key element in regional planning, particularly the development research and innovation strategies for smart specialization (RIS3) Europe. quadruple helix model from literature on systems been raised forefront RIS3. This comes spite fact that surrounding (i.e., collaboration among university, industry, government, civil society) is still its...

This study introduces a structured analysis of science parks as arenas designed to stimulate institutional collaboration and the commercialization of academic knowledge and technology, and the promotion of social welfare. A framework for key actors and their potential behaviour in this context is introduced based on the Triple Helix (TH) model and related literature. A link analysis was conduct...

Skeletal development and invasion by tumor cells depends on proteolysis of collagen by the pericellular metalloproteinase MT1-MMP. Its hemopexin-like (HPX) domain binds to collagen substrates to facilitate their digestion. Spin labeling and paramagnetic nuclear magnetic resonance (NMR) detection have revealed how the HPX domain docks to collagen I-derived triple helix. Mutations impairing tripl...

Advances in Internet of Things (IoT) devices and Machine Learning (ML) applications can provide valuable insights predictions on personal health by optimizing data generation processing. Nevertheless, the flow about status a patient brings variety technical, legal economic challenges that need to be addressed through an interdisciplinary approach. In this context, based action research methodol...

A 30 nt RNA with a sequence designed to form an intramolecular triple helix was analyzed by one-and two-dimensional NMR spectroscopy and UV absorption measurements. NMR data show that the RNA contains seven pyrimidine-purine-pyrimidine base triples stabilized by Watson-Crick and Hoogsteen interactions. The temperature dependence of the imino proton resonances, as well as UV absorption data, ind...

A minicollagen containing the COL1 and NC1 domains of chicken collagen XII has been produced in insect cells. Significant amounts of trimers contain a triple-helical domain in which the cysteines are not involved in inter- but in intrachain bonds. In reducing conditions, providing that the triple-helix is maintained, disulfide exchange between intra- and interchain bonding is observed, suggesti...

This case study reflects on a 2019 project involving staff-student partnership that focussed improving the international student experience at an Australian university. The responded to need for students feel sense of belonging and connectedness university as buffer against challenges they face are part acculturation process undergone upon arrival. project’s main output was creation short video...