Six transgenic tobacco lines, each homozygous for the beta-glucuronidase (GUS) gene at a different locus, and wild type were selfed and intercrossed to evaluate GUS activity in all possible hemizygous, homozygous and dihybrid combinations of GUS alleles. The transgenic lines are characterized by their GUS activity (two low, three intermediate, one high), T-DNA complexity (four single-copy, two ...

Monomeric Plum (Plum), a far-red fluorescent protein with photostability and photopermeability, is potentially suitable for in vivo imaging and detection of fluorescence in body tissues. The aim of this study was to generate transgenic cloned pigs exhibiting systemic expression of Plum using somatic cell nuclear transfer (SCNT) technology. Nuclear donor cells for SCNT were obtained by introduci...

in this study proline content and activity of catalase (cat), and ascorbate peroxidase (apx) and level of lipid peroxidation in terms of malondialdehyde (mda) content were measured in transgenic tobacco (nicotiana tabacum cv. wisconsin), over expressing a δ-1-pyrroline-5-carboxylate synthase (p5cs) gene, and non transgenic plants as control. drought stress was applied using polyethylene glycol ...

production of transgenic cotton (gossypium hirsutum l.) was studied utilizing agrobacterium-mediated strain lba4404 and pbi121 plasmid. this plasmid contains gus as a reporter gene driven by the camv35s promoter and nptii gene driven by nos as selection marker. in this study, the inheritance of gus gene was evaluated using selective media, pcr and gus staining in the second genertaion (t1) of t...

UNLABELLED The coxsackievirus and adenovirus receptor (CAR) is a cell contact protein with an important role in virus uptake. Its extracellular immunoglobulin domains mediate the binding to coxsackievirus and adenovirus as well as homophilic and heterophilic interactions between cells. The cytoplasmic tail links CAR to the cytoskeleton and intracellular signaling cascades. In the heart, CAR is ...

BACKGROUND Healing of burns is a complex process and very few effective treatments exist to facilitate the burn recovery process. Human acidic fibroblast growth factor 1 (FGF-1) plays an important role in a variety of biological processes, including angiogenesis, and tissue repair. Salvia miltiorrhiza is widely used in traditional Chinese medicine as an herb for the treatment of various disease...

Cotton (Gossypium hirsutum L.)  is an important fiber crop in Iran, cultivated on 150000-200000 ha of land.  In Iran the estimated loss due to the insect pest is more than 30%. Traditionally, pests are controlled by 10-12 times spraying per growing season of environmentally harmful chemical insecticides (e.g. endosulfan and/or methosystox). In order to produce transgenic cotton resistance to in...

Crowing-type chicken is one of the important chicken breeds in Indonesia. This study was conducted to investigate the crowing characters of Pelung chicken and the effect of exon 7 FoxP2 gene polymorphism in mute chicken. Chicken were obtained from local breeder. Pelung chicken crowing and morphology characterizations were conducted in Cianjur, West Java, Indonesia. Chicken breeding (♀ ...

We have previously demonstrated that antigen-specific T cell help can rescue mature Ig transgenic (Tg) hen egg lysozyme (HEL)-specific B cells from tolerance induction upon transfer into soluble HEL-expressing Tg hosts. Here we extend these findings by showing that T cell help could also rescue both immature and mature self-reactive B cells from rapid deletion in response to high-avidity membra...

Background: Lemongrass (Cymbopogon citratus) is an aromatic herb; rich source of citric, vitamin C, and phenolic compounds used as natural antioxidant and antibacterial agents in food processing. This study aimed to evaluate the effect of lemongrass extract on the quality properties of chicken burger during frozen storage. Methods: Lemongrass extract was prepared with three levels (0.5, 1.0, a...