To estimate the relative significance of exogenous vs. endogenous fatty acids in increasing hepatic triacylglycerol secretion following an inhibition of fatty acid oxidation by emeriamine, livers from 2-d-fasting rats were perfused with or without an inhibitor in the presence of a geometrical isomer of linoleate (linolelaidic acid, trans,trans-9,12-octadecadienoic acid). Emeriamine added to the...

Kinetics of lipase-catalyzed esterification of hexanoic acid and ethyl alcohol using the solvent-free system, surface coated lipase from Candida rugosa, had been studied. The effect of various parameters such as reaction time, reaction temperature, reaction kinetics, water removal and feasibility of solvent-free system had been focused. Candida Rugosa lipase was more effective than other li...

Porcine pancreatic lipase (PPL), a comparatively inexpensive enzyme, was used in the optical resolution of (R, S)ibuprofen in methanol through enantioselective esterification. The different reaction parameters during the esterification of (R, S)-ibuprofen using PPL was studied with respect to temperature, time, enzyme concentration, substrate concentration and water content. The percentage conv...

The livers of both baboons and rhesus monkeys fed a high fat, high cholesterol diet secreted very low density lipoproteins (VLDL) that were enriched in cholesteryl ester and apoe as compared to VLDL secreted by the livers of chow-fed animals. Stimulation of macrophage cholesterol esterification by the experimental VLDL was compared to that produced by the standard beta-VLDL obtained from the pl...

By DFT calculation, we found that acid-catalyzed carboxylic acid esterification and ester hydrolysis are brief two-step reactions. First, the carboxylic acid hydroxyl-oxygen or ester alkyl-oxygen is protonated, which generates a highly active acylium ion. The protonation requires an activation energy (Ea) of 4-10 kcal mol(-1), and is the rate-controlling step of the esterification or hydrolysis...

BACKGROUND Natural polysaccharides such as starch are becoming increasingly interesting as renewable starting materials for the synthesis of biodegradable polymers using chemical or enzymatic methods. Given the complexity of polysaccharides, the analysis of reaction products is challenging. RESULTS Esterification of starch with fatty acids has traditionally been monitored by saponification an...