ISSR markers were used to determine the genetic variation and sex determination of the three life-stages of 41 samples of Gracilaria corticata from two regions of the Persian Gulf and Oman Sea namely Bostaneh and Lipar. The specimens were cultured in PES media to observe the different life stages. DNA was extracted by modified CTAB method. After screening of 20 ISSR primers, four primers were s...

After the initial development of a human immunodeficiency virus type 1 (HIV-1) subtype-screening tool by nested multiplex PCR, we further improve it through the redesign of subtype-specific primers based on subtype signature pattern (SSP) analyses and optimization of the PCR conditions. Extracted RNA from plasma samples was used in reverse transcription and the cDNA products were added to the f...

background and objectives: dna ladder contains dna fragments of different length but with known size, used to determine the size of unknown dna molecules. different dna ladders are available for expected dna length. conserved sequences were selected for design of primers to generate dna fragments of known specific size. materials and methods: in this study, we describe a method by which dna lad...

Plasmodium vivax infections remain a major source of malaria-related morbidity and mortality. Early and accurate diagnosis is an integral component of effective malaria control programs. Conventional molecular diagnostic methods provide accurate results but are often resource-intensive, expensive, have a long turnaround time and are beyond the capacity of most malaria-endemic countries. Our lab...

The present study was conducted to investigate genetic diversity between and within of six populations with different individual numbers of cultivated and non-cultivated provenances of  Moringa peregrina (Forssk.) Fiori and Moringa oleifera Lam. using RAPD, ISSR and various combinations of RAPD and ISSR primers in one multiplex PCR (R-ISSR). 10 primers that produced clear and ...

Genus and Species. Sus scrofa. Locus. Pig serotonin receptor subtype 2 alpha ( HTR2A) and pig endothelin-B receptor ( EDNRB) . Source and Description of Primers. Heterologous primers for EDNRB were obtained from Leslie Lyons through the CATS project (Lyons et al., 1997) and primers for HTR2A were designed from highly conserved human (M86841) and mouse sequences (X72222). Primers ( EDNRB forward...

BACKGROUND AND OBJECTIVES DNA ladder contains DNA fragments of different length but with known size, used to determine the size of unknown DNA molecules. Different DNA ladders are available for expected DNA length. Conserved sequences were selected for design of primers to generate DNA fragments of known specific size. MATERIALS AND METHODS In this study, we describe a method by which DNA lad...

ustilago hordei (pres) lagerh is a fungus that causes covered smut of barley. this disease reduces yield and is a limiting factor in barley fields of iran. one hundred forty four isolates of u. hordei were collected from 36 regions of khuzestan province during 2009 and the genetic diversity of 100 isolates was evaluated by molecular markers. four out of 14 primers were selected on the basis of ...