Myxococcus xanthus fibrils are cell surface-associated structures composed of roughly equal amounts of polysaccharide and protein. The level of M. xanthus polysaccharide production under different conditions in the wild type and in several mutants known to have alterations in fibril production was investigated. Wild-type exopolysaccharide increased significantly as cells entered the stationary ...

The Escherichia coli K5 capsular polysaccharide (glycosaminoglycan) chains are composed of the repeated disaccharide structure: -GlcAbeta1,4-GlcNAcalpha1,4-(where GlcA is glucuronic acid and GlcNAc is N-acetyl-D-glucosamine). The GlcA, present in most glycosaminoglycans, is donated from UDP-GlcA, which, in turn, is generated from UDP-glucose by the enzyme UDP-glucose dehydrogenase (UDPGDH). The...

In order to extract the polysaccharide B of brucella used in Radial Immunodiffusion test (RID) for detection of infected cattle, a quick, simple and safe method is proposed. It consists of using phenol killed cells of B. melitensis 16 M known to produce polysaccharide B. After autoclaving brucella cells resuspended in saline, the cells are separated by centrifugation and the polysaccharide B is...

Pappagianis, D. (University of California, Berkeley), E. W. Putman, and G. S. Kobayashi. Polysaccharide of Coccidioides immitis. J. Bacteriol. 82:714-723. 1961.-Soluble polysaccharide from mycelia or culture filtrates of Coccidioides immitis was found to consist mainly of mannose, but also included small quantities of galactose and another reducing sugar. Isolation of the polysaccharide by etha...

A polysaccharide was found to be covalently linked to the peptidoglycan of the unicellular cyanobacterium Synechocystis sp. strain PCC6714 via phosphodiester bonds. It could be cleaved from the peptidoglycan-polysaccharide (PG-PS) complex by hydrofluoric acid (HF) treatment in the cold (48% HF, 0 degrees C, 48 h) yielding a pure, HF-insoluble peptidoglycan fraction and an HF-soluble polysacchar...

Plants with hypoglycemic properties are important in the treatment of diabetes. One of the mechanisms in reducing blood glucose is preventing the digestive absorption of glucose. The aim of this study was to evaluate the antioxidant properties of some traditional medicinal plants collected from different regions of Iran and their effects on glucose diffusion decrease. The amounts of phenolic co...

The chemical structure of a polysaccharide from the broth of cultured Streptomyces virginia H03 was investigated. There might be 1-->2 and 1-->4 and no 1-->6 glycosidic linkages in the polysaccharide according to periodate oxidation and Smith degradation. Four fragments including 2,3,4,6-Me4-Man, 2,3,6-Me3-Gal, 2,4,6-Me3-Glc, and 3,6-Me2-Man were found in the methylated polysaccharide. Furtherm...

The capsular polysaccharide of Diplococctw pneumoniae Type XII contains residues of D-glucose and D-galactose in a molar ratio of 2:l. The methylated polysaccharide yielded upon hydrolysis 2,3,4,6-tetraand 3,4,6-tri-O-methyl-D-glucose and 2,3,4,6-tetra-0-methyl-D-galactose as the only neutral methyl sugars. Periodate oxidation of the polysaccharide resulted in destruction of all neutral sugars ...

In this study, the efficient micropropagation protocol of Astragalus mongholicus Bunge was established and also triterpenoid saponin polysaccharide content in ethanol, methanol aqueous extracts different samples were determined by using spectrophotometric methods to investigate whether biologically active compounds depends on stage development plant during vitro culture. The total saponins poly...

‘Golden Delicious’ apples (Malus /domestica Borkh.) were either untreated or pressure-infiltrated after harvest with 0, 1, 2, 3 or 4% CaCl2 solutions (w/v) and stored at 0 8C for up to 6 months. The chemical composition of the fruit cortical tissue and cell walls of the 2 /4 mm layer under the epidermis was studied. Storage of untreated fruit resulted in a decrease in K, P, Mg, S and Suc conten...