BACKGROUND: Information on absorption and metabolism of strawberry polyphenols is limited. OBJECTIVE: The aim of this study was to characterize and quantify plasma polyphenols/anthocyanins across 4 doses of strawberry using Q-TOF LC/MS and LC-MS/MS analysis. METHOD: Plasma was collected from 5 subjects (n= 5) every 30–60 min for 6 h after consuming beverages containing 0, 10, 20 or 40 g freeze-...

Matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) fingerprinting is a fast and reliable method for the classification and identification of microorganisms, with applications in clinical diagnostics, environmental and taxonomical research, or food-processing quality control. The BioTyperTM MALDI-TOF MS fingerprinting system allows researchers to perform ...

We compared the diagnostic accuracy of the Carba NP test with that of a straightforward matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method for detecting carbapenemase-producing Enterobacteriaceae (CPE). Using PCR as the reference method, both tests demonstrated a sensitivity of 87% and a specificity of 100%. MALDI-TOF MS offers a potential alterna...

Colorectal cancer is the third most common cancer worldwide with an annual incidence of ~1 million cases and an annual mortality rate of ~655,000 individuals. There is an urgent need for identifying novel targets to develop more sensitive, reliable, and specific tests for early stage detection of colon cancer. Post-translational modifications are known to play an important role in cancer progre...

METHODS: We developed a broad-spectrum screening method on an ultra-performance liquid chromatography TOF mass spectrometer (UPLC-TOF-MS) using the MS mode. A diverse set of patient samples were subjected to a simple dilute, hydrolyze, and shoot protocol and analyzed in a blind manner. Data were processed with 3 sets of criteria with increasing stringency, and the results were compared with the...

We evaluated the usefulness of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for Cryptococcus identification at the species and subspecies levels by using an in-house database of 25 reference cryptococcal spectra. Eighty-one out of the 82 Cryptococcus isolates (72 Cryptococcus neoformans and 10 Cryptococcus gattii) tested were correctly identified w...