Solanesol is a noncyclic terpene alcohol that is composed of nine isoprene units and mainly accumulates in solanaceous plants, especially tobacco (Nicotiana tabacum L.). In the present study, RNA-seq analyses of tobacco leaves, stems, and roots were used to identify putative solanesol biosynthesis genes. Six 1-deoxy-d-xylulose 5-phosphate synthase (DXS), two 1-deoxy-d-xylulose 5-phosphate reduc...

BACKGROUND The introduction of pharmaceutical traits in tobacco for commercial production could benefit from the utilization of a transgene bioconfinement system. It has been observed that interspecific F1Nicotiana hybrids (Nicotiana tabacum × Nicotiana glauca) are sterile and thus proposed that hybrids could be suitable bioconfined hosts for biomanufacturing. We genetically tagged hybrids with...

Tobacco production is a key line in the Cuban economy and generates largest income agricultural sector. Within phytopathogens affecting this plant, Fusarium spp., constitutes pest of interest, due to vascular involvements that cause deterioration commercial value leaf. Therefore, research was carried out with aim determining antagonistic activity vitro native strains Trichoderma harzianum T. vi...

A transporter of the multidrug and toxic compound extrusion (MATE) family, Nicotiana tabacum MATE2 (NtMATE2), is located in vacuole membrane tobacco plant root involved transportation nicotine, a secondary or specialized metabolic Solanaceae. Here, we report crystal structures NtMATE2 its outward‐facing forms. The overall structure has bilobate V‐shape with pseudo‐symmetrical assembly N‐ C‐lobe...

The reproductive potentials of Heterodera glycines (mixture of races 3 and 4 and unidentified races) and a tobacco cyst nematode Globodera tabacum solanacearum were studied in the field. The experiments involved four cultivars of soybean Glycine max and four cultivars of Nicotiana tabacum. The reproductive potential of the H. glycines population was high on Essex and Lee 74 soybean but low on F...

The production of tobacco (Nicotiana tabacum L.) in Argentina is centered the northwestern region (NWA), where incidence root rots and stem diseases caused by Fusarium spp. has increased considerably recent years. This study aimed to evaluate pathogenicity levels isolates F. oxysporum solani complexes different varieties Virginia Type tobacco. commercial MB47, PVH229, NC71, K346, K326, K394 wer...

Pseudomonas tabaci (Wolf and Foster) Stevens, produces an exotoxin, detectable as a bacteriumfree, chlorotic halo, surrounding the necrotic focus of infection in the leaf of a susceptible host at the site of inoculation (Braun, 1955). Garber and Heggestad (1958) reported that species of Nicotiana and varieties of Nicotiana tabacum differed in their susceptibility, ranging from highly susceptibl...

Genetic engineering of plants has turned out to be an attractive approach produce various secondary metabolites. Here, we attempted kynurenine, a health-promoting metabolite, in Nicotiana tabacum (tobacco) transformed by Agrobacterium tumefaciens with the gene, coding for human indoleamine 2,3-dioxygenase 1 (IDO1), enzyme responsible kynurenine production because tryptophan degradation. The pre...