Treatment of solid and liquid tobacco wastes with high nicotine content remains a longstanding challenge. Here, we explored an environmentally friendly approach to replace tobacco waste disposal with resource recovery by genetically engineering Pseudomonas putida. The biosynthesis of 3-succinoyl-pyridine (SP), a precursor in the production of hypotensive agents, from the tobacco waste was devel...

Two photoproducts of DNA damage, i.e. cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4PPs), induced by UV-B radiation in suspension-cultured tobacco cells were quantified by enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies. CPDs and 6-4PPs were induced in tobacco cells by UV-B radiation. Photorepair of CPDs was faster than that of 6-4PPs. UV-B radiation induce...

background: histopathological diagnosis of lesions arising from the intake of tobacco is based on subjective evaluation of morphological alterations within the lesional tissue. oral exfoliative cytology is a non-invasive diagnostic technique for early detection of oral premalignant and malignant lesions. morphometric techniques have been advocated as objective and reproducible methods of detect...

the influence of salinity on the different polyamine fractions (free, conjugated, and bound) was compared in a natrophilic halophyte (beta vulgaris l. cv. ic) and a salt sensitive glycophyte (nicotiana rustica l. cv. basmas). low-level salinity (25 mm nacl) and high salinity (150 and 50 mm nacl for sugar beet and tobacco, respectively) were supplied in hydroponics. under low salinity shoot dry ...

PERIPHERAL SEAL OF ORAL DEFENSE LANGERHANS CELLS ANJALI NARWAL, POST GRADUATE INSTITUE OF DENTAL SCIENCES, U PT. BHAGWAT DAYAL SHARMA UNIVERSITY OF HEALTH SCIENCES, ROHTAK, HARYANA, INDIA Objective : Langerhans cells (LCs) form a network of immune competent cells in the epithelium of oral mucosa and alterations in their number on exposure to tobacco might impair mucosal immunologic protection a...

Objective: To evaluate the changes in the number of Langerhans Cells (LC) observed in the epithelium of smokeless tobacco (SLT-induced) lesions. Methods: Microscopic sections from biopsies carried out in the buccal mucosa of twenty patients, who were chronic users of smokeless tobacco (SLT), were utilized. For the control group, twenty non-SLT users of SLT with normal mucosa were selected. The ...

The tobacco extensin gene Ext 1.4 is expressed in cells submitted to mechanical constraints and in cells proliferating under hormone control