BACKGROUND Thyroglobulin mRNA can be detected qualitatively in the peripheral blood of patients with metastatic thyroid cancer, thyroid cancer patients with residual thyroid bed uptake, and individuals with no known thyroid disease with intact thyroid glands by use of a lengthy, highly sensitive extraction technique. To improve and broaden the clinical usefulness of this assay, we developed a q...

The availability of rat thyroglobulin cDNA clones was exploited to study the regulation of thyroglobulin gene transcription by thyrotropin (TSH). Groups of rats were subjected to treatments leading to reduction or increase in the rat serum TSH (rTSH) levels. Thyroid gland nuclei were isolated, incubated in vitro in the presence of 32P-labeled uridine triphosphate, and thyroglobulin transcripts ...

Thyroglobulin, the precursor of thyroid hormones, is extracellularly stored in a highly condensed and covalently cross-linked form. Solublization of thyroglobulin is facilitated by cysteine proteinases like cathepsins B and K which are proteolytically active at the surface of thyroid epithelial cells. The cysteine proteinases mediate the processing of thyroglobulin by limited extracellular prot...

Endogenously radioiodinated thyroglobulin (tg) and thyroxine (T4), in the circulation following, therapeutic doses of radioiodine were studied in five patients with differentiated thyroid carcinoma. Radioactive serum was fractionated on a Sephadex G-200 and the various radioactive peaks thus obtained were analyzed for their biochemical and immunologic characteristics using precipitation with tr...

Several studies have appeared in the past 25 years (l-5) concerning the physicochemical properties of thyroglobulin, the protein precursor of the thyroid hormone thyroxine. Most of this work has utilized hog thyroid gland as a source of material. The various investigations are in rather good agreement in obtaining a molecular weight for thyroglobulin close to -$ million. Moreover, this form of ...

Gostimir MIKAČ Faculty of Medicine Save Mrkalja 22 78000 Banja Luka Republic of Srpska Bosnia and Herzegovina [email protected] SUMMARY Introduction Thyroglobulin is composed glycoprotein, and it is synthesized by follicular cells of the thyroid gland. Treatment of differentiated thyroid carcinomas involves total thyroidectomy followed by radioiodine ablation of a potential remaining tissue. The m...

CONTEXT There has been a rise in the incidence of type 1 diabetes mellitus (T1DM) in India. The prevalence of thyroid autoantibodies and thyroid dysfunction is common in T1DM. AIMS The aim of this study is to determine the incidence of thyroid dysfunction and thyroid autoantibodies in T1DM subjects, without any history of thyroid disease, and the prevalence of glutamic acid decarboxylase (GAD...

The association of human T-lymphotropic virus type I (HTLV-I) with autoimmune disorders was investigated on the basis of prevalence of antinuclear antibody (ANA), rheumatoid factor and anti-thyroglobulin antibody as well as immunoglobulin (Ig) serum level (IgG, IgA, and IgM). The subjects, all atomic bomb survivors, were 59 HTLV-I-seropositive people without HTLV-I-associated myelopathy or adul...