objective: titration of viruses is important to determine the quantity of virus in vaccine development, master virus seed stock preparation, viral vector studies and virus replication. in this study, we compared the ccid50% and plaque assay as a standard titration method for rotavirus (rf) and hsv-1.   methods: the ma104 and vero cells were inoculated by rf and hsv-1 in 6- and 96-well plates. f...

A Microsoft Excel workbook has been developed to simplify the quantitative analysis of experimentally measured titration curves for diprotic and triprotic amino acids such as glycine, arginine, histidine, glutamate. Students perform titration, enter data into worksheet, manually adjust resulting pKa values achieve closest agreement between experimental calculated curves. At same time, students ...