Although bioprosthetic heart valves offer the benefits of a natural opening and closing, better hemodynamics, and avoidance of life-long anticoagulant therapy, they nevertheless tend to fail in 10-15 years from tears and calcification. Several authors, including the present ones, have identified the rigid stent as a factor contributing to these failures. The ultimate solution is an artificial h...

In a study of human milk collected for banking, heating at 62.5 degrees C for 30 min, and freezing and thawing resulted in hydrolysis of triglycerides. Freezing and thawing caused disruption of fat globules and a greater hydrolysis of triglycerides than did the heating process. There was a decrease in the percentage of the poly-unsaturated fatty acids linoleic acid (C18:2) and linolenic acid (C...

introduction: sperm cryopreservation is widely used as a way of preserving the male sperm used in future artificial insemination and fertilization. despite all the recent advances in cryopreservation technology, sperm motility is negatively affected by freezing and the thawing afterwards. yet hyaluronic acid is believed to improve the vitality and motility of the sperm, enhancing the physiologi...

Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells from a frozen stock have become important procedures performed in routine hES cell culture. Since hES cells are very sensitive to the stresses of freezing and thawing, special care must taken. Here we demonstrate the proper technique for rapidl...

Experiment I was designed to test the effect of thaw rate on semen packaged in glass ampules. One ejaculate from each of 10 Holstein bulls was diluted in egg yolk-citrate-glycerol, packaged in 1.0 ml glass ampules and frozen in liquid nitrogen vapor. Water bath thaws of 5 C for 10 rain, 20 C for 4 rain, 35 C f o r 4 min, 50 C for 75 sec, 65 C f o r 4 8 sec, 80 C for 37 sec and 100 C for 20 sec ...

Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and DNA integrity of rat spermatozoa during semen freeze-tha...

This study was carried out to obtain informations regarding the effect of N-acetyl-D-glucosamine in the LEY (lactoseegg yolk) diluent according to incubation time in 5 ml maxi-straw and the effects of freezing rate, thawing temperature and thawing time in the LEN (lactose-egg yolk and N-acetyl-D-glucosamine) diluent on acrosome morphology and motility of frozen-thawed boar sperm. The study show...

Background: Artificial insemination (AI) has been used for increasing genetic potential of animals in production while application of AI has been restricted in buffalo due to low freezing ability and fertility of frozen-thawed buffalo spermatozoa. So improvement in buffalo sperm cryopreservation may enhance the breeding programs of this valuable farm animal. Semen processing is one of the major...

Expansive soil is widely distributed in seasonally frozen areas worldwide. Due to the special expansion and shrinkage characteristics of expansive related water content, there are potential engineering disasters subgrade slope engineering. To investigate physical mechanical changes within slope, four freeze-thaw cycles tests were performed on models an environmental chamber with ratios 1:1.5, 1...