Most of the allergenic proteins from fruits identified so far belong to different families pathogenesis-related (PR) proteins. These PR have been classified in structurally and functionally unrelated proteins, but majority all fruit allergens three groups, particular PR-5 thaumatin-like (TLP), PR-10 Bet v 1-like PR-14 non-specific lipid transfer (nsTLP). Some can also be found among PR-protein ...

Sunflower leaf discs floated on a solution containing aspirin (acetylsalicylic acid) produced a set of new proteins extractable at pH 5.2 and excreted into the intercellular space. More than 80% of the proteins found in the intercellular fluids of induced leaf discs have been identified as pathogenesis-related (PR) proteins by their immunological relationship with tobacco PR proteins. Members o...

Anomalous scattering with soft X-ray radiation opens new possibilities in phasing for macromolecular crystallography. Anomalous scattering from S atoms collected on an in-house chromium radiation source (lambda = 2.29 A) was used to phase the X-ray diffraction data of thaumatin (22 kDa) and trypsin (24 kDa) crystals. The contribution to the anomalous term, Deltaf" = 1.14 e(-), from sulfur for C...

Along with the adoption of three-dimensional electron diffraction (3D ED/MicroED) as a mainstream tool for structure determination from sub-micron single crystals, questions about best practices regarding each step along workflow, data collection to solutions, arise. In this paper, we discuss three particular aspects 3D ED/MicroED experiment which, after hundreds structures solved in Rigaku’s l...

The cultivated almond displays a gametophytic self-incompatibility system, which avoids self-fertilization, and it is controlled by multi-allelic locus (S-locus) containing two genes specifically expressed in pistil (S-RNase) pollen (SFB). Studies on almonds with the same S-haplotype but different phenotype pointed to existence of unknown components this system explain its functioning. increase...

Peanut (Arachis hypogaea L) is one of the widely cultivated and leading oilseed crops of the world and its yields are greatly affected by various biotic and abiotic stresses. Arachis diogoi, a wild relative of peanut, is an important source of genes for resistance against various stresses that affect peanut. In our previous study a thaumatin-like protein gene was found to be upregulated in a di...

Thaumatin-like proteins (TLPs) are induced by a variety of phytopathogens in many plants and several TLPs are allergenic. Previously, we isolated three TLP-encoding cDNAs (Cry j 3.1, Cry j 3.2 and Cry j 3.3) from a cDNA library derived from the pollen of Cryptomeria japonica D. Don. Here, we describe three new TLP cDNAs (Cry j 3.4, Cry j 3.5 and Cry j 3.6). We compared the sequences, the geneti...