The most reliable method for Rhesus group determination is probably the standard tube method (Landsteiner and Wiener, 1941; Boorman, Dodd, and Mollison, 1942; Taylor, 1943). This technique has only one serious disadvantage: it may take as long as two hours to perform, although occasional high-avidity anti-D sera will give clearcut results in 30 minutes or even less. For emergency Rhesus typing ...

BACKGROUND Staphylococcus aureus is often responsible for fatal infections and recent upsurge of resistant strains has resulted in therapeutic failure. The identification of this microorganism is a major challenge to medical microbiologists in developing countries. METHODS One hundred and eighty five isolates which had been previously isolated from the nares of 185 healthy college students' v...

To provide references for the fire prevention design of steel–concrete–steel immersed tube tunnels, four types test conditions—no protection, fireproof coating insulation, single-layer seam boards, and double-layer boards—were carried out using partial full-size structural members. Additionally, thermal insulation effects various fireproofing technology solutions were contrasted analyzed. Combi...

OBJECTIVE To evaluate the influence of pleural drainage on the distance covered on the six-minute walk test, pain intensity and vital capacity in patients submitted to pulmonary resection. METHODS Thirteen consecutive patients from the Thoracic Surgery Infirmary of Hospital São Paulo, Brazil, submitted to closed pleural drainage (0.5-in multiperforated chest tube) in the postoperative period ...

Standard broth microdilution (with and without bovine serum albumin [BSA] supplementation), tube dilution, and agar dilution susceptibility tests were compared for determining ramoplanin MICs. With a data base of 246 clinical isolates of gram-positive bacteria from 33 U.S. sites, it was shown that (i) agar and tube dilution susceptibility tests gave essentially the same results (93.9% of the te...

Posttranslational modifications of histones have been strongly correlated with transcriptional regulation. In this issue of Cell, comprehensively examined the nature of arginine methyltransferases and histone modifications in p53-mediated transcription.

For most living things, 24,000 generations is a daunting span of time. Go back that many human generations, or about 500,000 years, and Homo sapiens had not yet evolved. Even for the fruit flies beloved of geneticists, 24,000 generations equals about 1500 years. But in Richard Lenski's laboratory at Michigan State University in East Lansing, 24,000 generations ago is a recent memory. The year w...

The eukaryotic nucleotide excision-repair pathway has been reconstituted in vitro, an achievement that should hasten the full enzymological characterization of this highly complex DNA-repair pathway.