background: designing novel therapeutic agents has been a critical challenge for hiv disease. materials and methods: in current study a dna sequence which was encoded the tat protein was synthesized and inserted in pet 28 vector. vector was cloned in bl21-de3 e. coli and cultured in tb media. after protein expression, recombinant tat protein was purified by nta affinity chromatography. the tat ...

21 TNM Staging of Non–Small Cell Lung Cancer: Comparison of PET/MR and PET/CT Martin W. Huellner, Felipe de Galiza Barbosa, Lars Husmann, Carsten M. Pietsch, Cäcilia E. Mader, Irene A. Burger, Paul Stolzmann, Gaspar Delso, Thomas Frauenfelder, Gustav K. von Schulthess, and Patrick Veit-Haibach 27 Phase I Study of Ga-HER2-Nanobody for PET/CT Assessment of HER2 Expression in Breast Carcinoma Marl...

Nanobodies are recombinant, antigen-specific, single-domain, variable fragments of camelid heavy chain-only antibodies. The innate supremacy of nanobodies as a renewable source of affinity reagents, together with their high production yield in a broad variety of expression systems, minimal size, great stability, reversible refolding and outstanding solubility in aqueous solutions, and ability t...

The discovery of naturally occurring, heavy-chain only antibodies in Camelidae, and their further development into small recombinant nanobodies, presents attractive alternatives in drug delivery and imaging. Easily expressed in microorganisms and amenable to engineering, nanobody derivatives are soluble, stable, versatile, and have unique refolding capacities, reduced aggregation tendencies, an...

Post-translational modifications are difficult to visualize in living cells and are conveniently analyzed using antibodies. Single-chain antibody fragments derived from alpacas and called nanobodies can be expressed and bind to the target antigenic sites in living cells. As a proof of concept, we generated and characterized nanobodies against the commonly used biomarker for DNA double strand br...

Significance Neutralizing antibodies are important for immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and as therapeutics the prevention treatment of COVID-19. We identified high-affinity nanobodies SARS-CoV-2 receptor-binding domain found that nanobody cocktails consisting two noncompeting were able to block ACE2 engagement with RBD variants present in human popu...

Abstract Background The widespread popularity of porcine circovirus type 2(PCV2) has seriously affected the healthy development pig industry and caused huge economic losses worldwide. A rapid reliable method is required for epidemiological investigation evaluating effect immunization. However, current methods PCV2 antibody detection are time-consuming or very expensive rarely meet requirements ...