نتایج جستجو برای: synthetic promoter

تعداد نتایج: 228934  

Journal: :Cell 2012
Ahmad S. Khalil Timothy K. Lu Caleb J. Bashor Cherie L. Ramirez Nora C. Pyenson J. Keith Joung James J. Collins

Eukaryotic transcription factors (TFs) perform complex and combinatorial functions within transcriptional networks. Here, we present a synthetic framework for systematically constructing eukaryotic transcription functions using artificial zinc fingers, modular DNA-binding domains found within many eukaryotic TFs. Utilizing this platform, we construct a library of orthogonal synthetic transcript...

Journal: :Science 2017
Yi Wu Bing-Zhi Li Meng Zhao Leslie A Mitchell Ze-Xiong Xie Qiu-Hui Lin Xia Wang Wen-Hai Xiao Ying Wang Xiao Zhou Hong Liu Xia Li Ming-Zhu Ding Duo Liu Lu Zhang Bao-Li Liu Xiao-Le Wu Fei-Fei Li Xiu-Tao Dong Bin Jia Wen-Zheng Zhang Guo-Zhen Jiang Yue Liu Xue Bai Tian-Qing Song Yan Chen Si-Jie Zhou Rui-Ying Zhu Feng Gao Zheng Kuang Xuya Wang Michael Shen Kun Yang Giovanni Stracquadanio Sarah M Richardson Yicong Lin Lihui Wang Roy Walker Yisha Luo Ping-Sheng Ma Huanming Yang Yizhi Cai Junbiao Dai Joel S Bader Jef D Boeke Ying-Jin Yuan

Debugging a genome sequence is imperative for successfully building a synthetic genome. As part of the effort to build a designer eukaryotic genome, yeast synthetic chromosome X (synX), designed as 707,459 base pairs, was synthesized chemically. SynX exhibited good fitness under a wide variety of conditions. A highly efficient mapping strategy called pooled PCRTag mapping (PoPM), which can be g...

Promoter methylation is one of the main epigenetic mechanisms that lead to the inactivation of tumor suppressor genes during carcinogenesis. Due to the reversible nature of DNA methylation, many studies have been performed to correct theses epigenetic defects by inhibiting DNA methyltransferases (DNMTs). In this case novel therapeutics especially siRNA oligonucleotides have been used to specifi...

2003
Patrick Dunant Hanns Lochmüller

Expression of mini-dystrophin driven by the 1.35 kb MCK promoter ameliorates muscular dystrophy in fast, but not in slow muscles of transgenic mdx mice. Gentamicin fails to increase dystrophin expression in dystrophin-deficient muscle. (2003) U7 snRNAs induce correction of mutated dystrophin pre-mRNA by exon skipping. Antibody-mediated targeting of an adenovirus vector modified to contain a syn...

2006
Bruce D. Whitaker Henry A. Wallace Steven W. Pechous Christopher B. Watkins

Objectives: (1) Obtain complete genomic clones plus promoter regions of apple peel genes encoding HMGR isozymes HMG2 and HMG3 and determine whether expression of these genes is regulated by ethylene; (2) Demonstrate function of the gene product and obtain a genomic clone of the ethyleneresponsive promoter of the putative apple α-Farnesene Synthase gene; (3) Determine correlation among ethylene-...

2010
Marcy J. Balunas Douglas Kinghorn

Several synthetic aromatase inhibitors are currently in clinical use for the treatment of postmenopausal women with hormone-receptor positive breast cancer. However, these treatments may lead to untoward side effects and so a search for new aromatase inhibitors continues, especially those for which the activity is promoter-specific, targeting the breast-specific promoters I.3 and II. Recently, ...

Journal: :The Journal of biological chemistry 2002
Huei-Ru Lo Cheng-Chung Chou Tzong-Yuan Wu Joyce Pui-Yee Yuen Yu-Chan Chao

A DNA sequence upstream from the polyhedrin gene of baculovirus Autographa californica nucleopolyhedrovirus (AcMNPV) was found to activate strongly the expression of full or minimal promoters derived from AcMNPV and other sources. Promoters tested included the minimal CMV (CMVm) promoter from human cytomegalovirus, the full heat shock 70 promoter from Drosophila, and the minimal p35 promoter fr...

Journal: :Planta medica 2010
Marcy J Balunas A Douglas Kinghorn

Several synthetic aromatase inhibitors are currently in clinical use for the treatment of postmenopausal women with hormone-receptor positive breast cancer. However, these treatments may lead to untoward side effects and so the search for new aromatase inhibitors continues, especially those for which the activity is promoter-specific, targeting the breast-specific promoters I.3 and II. Recently...

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