Degradative enzymes have been used to obtain defined fragments of the isolated cell walls of suspension-cultured sycamore cells. These fragments have been purified and structurally characterized. Fragments released from endopolygalacturonase-pretreated cell walls by a purified endoglucanase and the fragments extracted from these walls by urea and alkali provide evidence for a covalent connectio...

Fluid phase endocytic uptake of external solutes in plant cells was further substantiated using artificial polystyrene nano-spheres (40 nm) and CdSe/ZnS quantum dots (20 nm). Both types of artificial nano-particles were taken up by sycamore-cultured cells. However, whereas polystyrene nano-spheres were delivered to the central vacuole, CdSe/ZnS nano-dots were sequestered into cytoplasmic vesicu...

Proteins extracted from the cell walls of Red Kidney bean hypocotyls, tomato stems, and suspension-cultured sycamore cells can completely inhibit the activity of the polygalacturonases (polygalacturonide hydrolases, EC 3.2.1.15) secreted by the fungal plant pathogens Colletotrichum lindemuthianum, Fusarium oxysporum, and Sclerotium rolfsii. The inhibitor of the C. lindemuthianum polygalacturona...

FREQUENCY GPR AND OTHER ELECTROMAGNETIC SOUNDING TECHNIQUES. S. M. Clifford, E. Heggy, M. Ali, V. Ciarletti, C. Corbel, C. L. Dinwiddie, F. Dolon, A. Le Gall, R. E. Grimm, R. N. McGinnis, R. Ney, S. K. Sandberg Lunar and Planetary Institute, 3600 Bay Area Blvd., Houston, TX 77058, e-mail: [email protected]; Department of Astronomy, Cairo University; Egyptian Geological Survey and Mining Aut...

Human migrations across geographic boundaries can facilitate the introduction of new husbandry practices and dispersal of plants and animals, resulting in changes in biodiversity. As previously demonstrated, the 12th century BCE Philistine migration-to the southern Levantine littoral, involved the transportation of pigs from Europe, engendering long term genetic displacement of local Near Easte...

Degradation of the sycamore cell wall by hydrazinolysis yielded soluble glycopeptides. The peptide portion of the predominant glycopeptide was shown to be cyclic (4-trans-hydroxy-l-prolyl)-4-trans-hydroxy- l-proline. From this structure and the composition of the major glycopeptide it was deduced that only short oligosaccharides are attached to the hydroxyprolyl residues of the wall glycoprotei...

This paper describes the isolation and characterization of rhamnogalacturonan II, a hitherto unobserved component of the primary ceo wails of dicotyledonous plants. Rhamnogalacturonan II constitutes 3 to 4% of the primary ceo wais of suspension-cultured sycamore (Acerpseudoplatanus) cels. Rhamnogalacturonan II is a very complex polysaccharide yielding, upon hydrolysis, 10 different monosacchari...