A new amplified enzyme linked immunosorbent assay (amplified ELISA) kit for detecting herpes simplex virus (HSV) antigen was evaluated. Duplicate swabs were taken from 180 patients with clinically suspected herpes lesions. Tests were performed on a direct swab extract and viral transport medium containing a swab. Of the 93 culture positive specimens, 78 of the extracted samples (sensitivity 83....

BACKGROUND Tonsillitis is one of the most common childhood afflictions. This prospective study was designed to explore the possible role of fine needle aspiration of recurrently inflamed tonsils in determining their core flora. RESULTS Seventy-seven patients at DHQ Hospital Haripur undergoing tonsillectomy for recurrent tonsillitis were included in the study. Colonies grown from fine needle a...

Chickens were experimentally infected with Salmonella thompson (serogroup C, paratyphoid) and Salmonella pullorum (serogroup D). Five serological methods and one cultural method were used in detecting the infections. The microantiglobulin test was superior to all other methods for detection of paratyphoid (S. THOMPSON) infection and was followed in efficacy by the microagglutination test, rapid...

MATERIALS Reagents Dithiothreitol (DTT), 1 M DNA IQ System (Promega; includes Resin, Lysis Buffer, 2X Wash Buffer, and Elution Buffer) Ethanol, 95-100% Isopropanol Sample (liquid blood, cotton swab, th CEP swab, 15-50 mm2 S&S paper, 3-30 mm2 FTA paper) Equipment Aerosol-resistant micropipette tips DNA IQ Spin Baskets (Promega) Heating blocks, preset to 65°C and 95oC MagneSphere Technology Magne...

Diabetic foot ulcers and osteomyelitis may limit health-related quality of life and contribute to mobility impairment, treatment morbidity, amputation, and death [1–6]. The ulcer may be caused by minor or repetitive trauma to the neuropathic foot, and bacterial seeding may result in a chronic draining sinus, osteomyelitis, and secondary bony deformity. Diagnosis of osteomyelitis in the diabetic...

The fully automated COBAS AMPLICOR CT/NG and semiautomated AMPLICOR CT/NG tests were evaluated in a multicenter trial for their ability to detect Neisseria gonorrhoeae infections. Test performance compared to that of culturing was evaluated for 2,192 matched endocervical swab and urine specimens obtained from women and for 1, 981 matched urethral swab and urine specimens obtained from men. Cult...

BACKGROUND Our previous study showed that cotton-swab cryotherapy is an alternative treatment modality for oral leukoplakia. METHODS This study used liquid nitrogen spray with a cryogun (cryogun cryotherapy) to treat 60 oral leukoplakia lesions. RESULTS Complete regression was achieved in all 60 oral leukoplakia lesions after cryogun cryotherapy. We found that 60 oral leukoplakia lesions tr...

Four varieties of swabs held in plastic containers and three swab transport media kits, all obtainable in the United Kingdom, were tested to assess their effectiveness in the isolation of Bordetella pertussis. The highest isolation rates up to 48 hours were obtained with Dacron swabs held in plastic containers. Swab-transport media kits proved unsatisfactory. B pertussis may have a stronger aff...

A thorough understanding of virus diversity in wildlife provides epidemiological baseline information about pathogens. In this study, eye swab samples were obtained from semi-domesticated reindeer (Rangifertarandus tarandus) in Norway during an outbreak of infectious eye disease, possibly a very early stage of infectious keratoconjunctivitis (IKC). Large scale molecular virus screening, based o...

The objective of the present study was to isolate and identify Clostridium chauvoei from swab (n=750) and tissue (n=50) samples from cattle suspected for black quarter in six districts belonging to different climatic zones (Punjab, Pakistan). Conventional culture methods showed 192(25.6%) and 19(38%) positive from swab and tissues respectively for this bacterium. These bacterial isolates were f...