The identification of cancer stem cells in vivo and in vitro relies on specific surface markers that should allow to sort cancer cells in phenotypically distinct subpopulations. Experiments report that sorted cancer cell populations after some time tend to express again all the original markers, leading to the hypothesis of phenotypic switching, according to which cancer cells can transform sto...

since alkaline substances can rapidly penetrate into the cornea and subsequently damage limbal stem cells, another source of stem cells may be necessary to reconstruct the ocular surface. omentum has some such characteristics like ability to regenerate tissue as well as anti-inflammatory capacity. presence of adult stem cells and pluripotent embryonic cell markers make it suitable in wound heal...

background: radiation-induced molecular changes on the endothelial surface of brain arteriovenous malformations (avm) may be used as markers for specific vascular targeting agents. in this study, we examined the level of expression of platelet endothelial cell adhesion molecule-1 (pecam-1) on brain endothelial cell surface after radiation treatment, with the aim of targeting the radiation-induc...

A second surface antigen, B(L), lethal in the presence of specific antibody and complement has been identified on some human cells and shown to behave as a good genetic marker. It is autosomal, unlinked to the human A(L) antigen previously described, and unlinked to 15 other human genes. The A(L) antigen, which is linked to the lactic dehydrogenase A gene, is found on the HeLa, the cultured hum...

Biosensors based on phage display-derived peptides as biorecognition molecules were used for the detection of cell surface cross-species markers in tissue homogenates. The peptide selected for murine myofibers was immobilized onto the surface of an acoustic wave sensor by biotin-streptavidin coupling. To detect peptide-receptor interaction, the sensors were exposed to muscle and control (kidney...

We recently identified genes encoding tumor endothelial markers (TEMs) that displayed elevated expression during tumor angiogenesis. From both biological and clinical points of view, TEMs associated with the cell surface membrane are of particular interest. Accordingly, we have further characterized four such genes, TEM1, TEM5, TEM7, and TEM8, all of which contain putative transmembrane domains...

PURPOSE Transplantation of cells into retinas affected by degenerative diseases to replace dying photoreceptors represents a promising therapeutic approach. Young photoreceptors of 4-day-old mice show the highest capacity to integrate into the retinas of adult mice following grafting. Additional enrichment of these donor cells before transplantation with cell surface marker-dependent sorting me...

Glycosylation of proteins and lipids on cell surface have been shown to be important in maintaining pluripotency and stem cell fate in embryonic stem cells. Lectins have been widely used to characterize carbohydrate modifications on cell surface of embryonic stem cells to determine pluripotency and stem cell fate. In the present study, a panel of 14 lectins and carbohydrate antibodies was used ...

Human peripheral lymphocytes were investigated for receptors binding Epstein-Barr virus (EBV) because of the regular association of this virus with infectious mononucleosis and Burkitt's lymphoma. This was done by a cytoadherence technique where virus-producing cells, displaying fresh viral determinants in their cytoplasmatic membrane, were mixed with lymphocytes. Unfractionated lymphocytes wer...

purpose: to describe a novel surgical technique for in vivo limbal stem cell cultivation for reconstruction of corneal epithelium in total limbal stem cell deficiency (lscd). methods: patients suffering from chronic total unilateral lscd due to a chemical injury were sequentially included. one small limbal block (2x1 mm) harvested from contralateral healthy eye sutured over the amniotic membran...