نتایج جستجو برای: subtilisin
تعداد نتایج: 2234 فیلتر نتایج به سال:
Two peptides containing the desmosine cross-link were isolated and purified from a subtilisin digest of oxalic acidsolubilized elastin. Molecular weights averaged approximately 6000. Automated sequence data suggest that the desmosines cross-link two polypeptide chains and that both desmosine and isodesmosine are present in the same primary sequence approximately equally substituted. The primary...
We introduce a computational method to optimize the in vitro evolution of proteins. Simulating evolution with a simple model that statistically describes the fitness landscape, we find that beneficial mutations tend to occur at amino acid positions that are tolerant to substitutions, in the limit of small libraries and low mutation rates. We transform this observation into a design strategy by ...
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