The enhanced synthesis of fatty acids in the liver and adipose tissue in response to insulin is critically dependent on the transcription factor SREBP-1c (sterol-regulatory-element-binding protein 1c). Insulin increases the expression of the SREBP-1c gene in intact liver and in hepatocytes cultured in vitro. To learn the mechanism of this stimulation, we analysed the activation of the rat SREBP...

Obesity-induced inflammation is crucial in the pathogenesis of insulin resistance and type 2 diabetes. In this study, we investigated effects Gracilaria chorda (GC) on lipid accumulation obesity-induced inflammatory changes or glucose homeostasis cell models (3T3-L1 adipocytes RAW 264.7 macrophages). Samples GC were extracted using solvents (water, methanol, ethanol) subcritical water (SW) at d...

In chick embryo hepatocytes, activation of acetyl-CoA carboxylase-alpha (ACCalpha) transcription by 3,5,3'-triiodothyronine (T3) is mediated by a cis-acting regulatory unit (-101 to -71 bp) that binds the nuclear T3 receptor (TR) and sterol regulatory element-binding protein-1 (SREBP-1). SREBP-1 directly interacts with TR on the ACCalpha gene to enhance T3-induced transcription. Here, we show t...

Celiac disease (CD) is a systemic immune-mediated disorder caused by the dietary gluten in individuals who are genetically susceptible to the disease. In fact, CD is a T cell-mediated immune disease in which gluten-derived peptides activate the lamina propria CD4+ Teff cells, and these T-cell subsets can cause the intestinal tissue damages. Also, there are additional subsets of CD4+ T cells wit...

Oxygenated derivatives of cholesterol, or oxysterols, regulate cholesterol homeostasis by interacting directly with Insig proteins and promoting both endoplasmic reticulum (ER) retention of transcription factors that control expression of cholesterol synthesis genes and degradation of the cholesterol biosynthetic enzyme HMG CoA reductase (HMGR). Gale et al. used the side chain oxy­ sterol 25­hy...

MicroRNA (miRNA or miR) inhibition of oncogenic related pathways has been shown to be a promising therapeutic approach for cancer. Aberrant lipid and cholesterol metabolism is involved in prostate cancer development and progression to end-stage disease. We recently demonstrated that a key transcription factor for lipogenesis, sterol regulatory element-binding protein-1 (SREBP-1), induced fatty ...

JNJ-26854165 (serdemetan) has previously been reported to inhibit the function of the E3 ligase human double minute 2, and we initially sought to characterize its activity in models of mantle cell lymphoma (MCL) and multiple myeloma (MM). Serdemetan induced a dose-dependent inhibition of proliferation in both wildtype (wt) and mutant (mut) p53 cell lines, with IC50 values from 0.25 to 3 mM/l, i...