Effects of subcultivation of human bone marrow mesenchymal stem cells on their capacities for chondrogenesis and supporting hematopoiesis, and telomea length were investigated. Mesenchymal stem cells were isolated from human bone marrow aspirates and subcultivated several times at 37 degrees C under a 5% CO(2) atmosphere employing DMEM medium containing 10% FCS up to the 20th population doublin...

CD34 is expressed on human hematopoietic stem and progenitor cells, and its clinical usefulness for the purification of stem cells has been well established. However, a similar pattern of expression for murine CD34 (mCD34) has not yet been determined. Two polyclonal anti-mCD34 antibodies that specifically recognize both endogenous and recombinant murine CD34 were daveloped to characterize the m...

Surface modification of medical polymers is carried out to improve biocompatibility. In this study, conventional polyurethane was exposed to microwave plasma treatment with oxygen and argon gases for 30 seconds and 60 seconds. Attenuated total reflection Fourier transform infrared spectra investigations of irradiated samples indicated the presence of functional groups. Atomic force microscope i...

chemical and morphological characterization of different parts of banana plant(musa paradisica) (peduncle, leaf stalk and pseudo stem) were carried out in orderto assess their potentiality for chemical pulp. each portion was tested forextractives, sodium hydroxide solubility, ash, lignin, cellulose and pentosancontents as well as their fiber length and width. these segmented parts werecharacter...

BACKGROUND Limited resources for adult stem cells necessitate their in vitro culture prior to clinical use. Investigating mitochondrial DNA (mtDNA) and telomere shortening has proved to be important indications of stem cell validity. This study was designed to investigate these indicators in multiple passages of three adult stem cell lines which were produced in our stem cell laboratory. METH...

Abstract Studies of education and careers in science, technology, engineering, math (STEM) commonly use a pipeline metaphor to conceptualize forward movement persistence. However, the “STEM pipeline” carries implicit assumptions regarding length (i.e. that it “starts” “stops” at specific stages one’s or career), contents some occupational fields are “in” while others not), perceived purpose “le...

to investigate effect of calcium and growth medium on lilium (asiatic hybrid lilium bulbs “tresor” cultivar) and nutrients uptake, a factorial experiment based on completely randomized design was conducted by three levels of calcium in nutrient solution (2, 4 and 6 mm*) and five growth media (100% perlite, 70% perlite + 30% cocopeat, 50% perlite + 50% cocopeat, 30% perlite + 70% cocopeat, 100% ...

in order to study the realationship between genetic diversity and different characteristics associated with grain yield in barley, 28 genotypes of barley cultivars contain seven cultivars and 21 hybrids were evaluated in randomized complete block (rcb) design with three replications. cultivars were valfajr, makuei, kavir, rayhan, gorgan, nusrat and nimroz. this study was carried out in research...