Abbreviations: GBC: Gallbladder Cancer; PCR: Polymerase Chain Reaction; SSCP: Single Strand Conformation Polymorphism; MSI: Microsatellite Instability; CIS: Carcinoma In Situ; AJPBD: Anomalous Junction of Pancreatico Biliary Duct; FHIT: Fragile Histidine Triad; CC: Chronic Cholecystitis; XGC: Xanthogranulomatous Cholecystitis; SAGE: Serial Analysis of Gene Expression; CTGF: Connective Tissue Gr...

SUPPLEMENTAL MATERIALS & METHODS: TABLE OF CONTENTS I. PRIMARY DATA GENERATION .........................................................................................3 Cell Culture and Cell Lines Assayed .....................................................................................................................................3 Sub-cellular Fractionation and RNA Isolation (CSHL)......

We investigated gene expression associated with trophectoderm epithelial intercellular junction formation in single human embryos at different stages of cleavage using RT-PCR methods based upon magnetic bead separation of polyA+ RNA. Trophectoderm tight junction (TJ) and desmosome biogenesis contribute to intercellular sealing and tissue integrity, critical for vectorial transport and blastocoe...

MOTIVATION The Affymetrix Human Exon Junction Array is a newly designed high-density exon-sensitive microarray for global analysis of alternative splicing. Contrary to the Affymetrix exon 1.0 array, which only contains four probes per exon and no probes for exon-exon junctions, this new junction array averages eight probes per probeset targeting all exons and exon-exon junctions observed in the...

Interneuronal gap junctional coupling is a hallmark of neural development whose functional significance is poorly understood. We have characterized the extent of electrical coupling and dye coupling and patterns of gap junction protein expression in lumbar spinal motor neurons of neonatal rats. Intracellular recordings showed that neonatal motor neurons are transiently electrically coupled and ...

Coupling of neurons by electrical synapses (gap junctions) transiently increases in the mammalian CNS during development. We report here that the developmental increase in neuronal gap junction coupling and expression of connexin 36 (Cx36; neuronal gap junction protein) are regulated by an interplay between the activity of group II metabotropic glutamate receptors (mGluRs) and GABA(A) receptors...

background: one million people are diagnosed with gastric cancer (gc) annually and the prognosis for patients with advanced gc or gastroesophageal junction (gej) cancer is usually poor with a median survival time of 8 to 10 months. recent reports have identified that anti her2 target therapy improved the median survival rate in patients with her2 positive gc; but a wide range of her2 overexpres...

Rapid pathogen testing is vital to the food industry. Enzyme immunoassays (EIA) provide reliable negative results in 48 h, but a presumptive positive (suspect) EIA result must be confirmed by traditional culture methods, requiring an additional 72 h. Polymerase chain reaction (PCR) testing technology is accepted as an accurate diagnostic tool. However, traditional PCR techniques can require sev...

Highly Sensitive and Multiplexed Quantification of mRNA Splice Variants by Direct Ligation of DNA Probes at Exon Junction and Universal PCR Amplification Honghong Wang,‡ Hui Wang,‡ Xinrui Duan,* Yuanyuan Sun, Xiangdong Wang and Zhengping Li* Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province; School of Chemical and Chemical Engineering, Shaanxi Normal University, Xi’an ...

This vignette briefly describes the content in the seqc package. The seqc package provides a series of data frames derived from the SEQC project between 2011 to 2014. Three types of data frames are included in this package: RNA-seq read count tables, junction tables and an additional gene intensity table generated by using the TaqMan RT-PCR technology. All the data frames are ready to use in th...