Cyclodextrin (CD) is synthesized by bacterial cyclodextrin glycosyltransferase (CGTase) and is widely used in food, pharmaceutical, cosmetic, and agricultural industries. In this study, Bacillus circulans CGTase was partially purified by ammonium sulfate precipitation at 50-70% saturation. The optimum pH and temperature for CD production from sago starch were found to be in the ranges of 4.5-5....

Starch, total amylolytic and phosphorylase activities were determined in lentil cotyledons during the first days of germination. Several independent criteria show that the amylolytic activity is due mainly to an amylase of the alpha type. Starch is degraded slowly in the first days; during this time, alpha- and beta-amylase activity are very low, while phosphorylase increases and reach a peak o...

A procedure was developed for the separation and purification of amylose and amylopectin isolated from cotton leaves. Cotton leaves were homogenized in 0.02 molar phosphate buffer at pH 7.0 containing HgCl(2) plus toluene. Crude starch granules were collected by centrifugation and partially purified by treating with acetone and toluene. The starch granules were then dispersed in dimethylsulfoxi...

The enzymatic and acid hydrolysis have converted eight new starches into a range of chain lengths mainly including glucose, maltose, and maltodextrins as observed on TLC plates, irrespective to the starch variety and treatment. Results of the enzymatic hydrolysis have highlighted the possibility of the use of V4 and V64, which can be labelled as "dietary fibres", to enhance the organoleptic qua...

The effect of a traditional Ethiopian lupin processing method on the chemical composition of lupin seed samples was studied. Two sampling districts, namely Mecha and Sekela, representing the mid- and high-altitude areas of north-western Ethiopia, respectively, were randomly selected. Different types of traditionally processed and marketed lupin seed samples (raw, roasted, and finished) were col...

Apparent digestibility coefficients (ADCs) of dry matter, crude protein, crude lipid, nitrogen-free extract, and energy in selected carbohydrate sources including wheat flour (WF), α-potato starch (PS), α-corn starch (CS), Na alginate (AL), dextrin (DEX), and carboxymethyl cellulose (CMC) were determined for olive flounder. The olive flounder averaging 150 ± 8.0 g were held in 300-L tanks at a ...

An experiment was conducted with 72 Bovans Brown laying hens to determine the effect of dietary crude glycerin on laying performance; egg quality; retention of N, Ca, and P; and metabolizability of energy. The dietary treatments consisted of a control corn-soybean diet containing 6% corn starch (17% CP, 2,775 kcal/kg of AME(n), 0.81% lysine, 0.36% methionine, 3.60% Ca, and 0.37% available P) an...

The subcellular localization of the starch biosynthetic and degradative enzymes of spinach leaves was carried out by measuring the distribution of the enzymes in a crude chloroplast pellet and soluble protein fraction, and by the separation on sucrose density gradients of intact organelles, chloroplasts, peroxisomes, and mitochondria of a protoplast lysate. ADP-Glucose pyrophosphorylase, starch...

Walnut (Juglans regia L.) were evaluated for some physical properties and chemical composition. Ripe fruits were determined for moisture (2.71 %), crude protein (14.6 %) crude fibre (1.8 %) ash (1.57 %), crude energy (576 Cal) ether soluble extract (24.71 %), nut (10.5 g) and kernel (5.09 g) veights. Relative density, refractive index, free fatty acids, peroxide value, saponification number, un...

The phytochemical and proximate compositions of the dried leaves of Gongronema latifolium were analyzed using the methods of Association of Official Analytical Chemists (A.O.A.C). The results of proximate composition showed that the leaves contained moisture (11.13±0.01%), crude protein (33.60±0.00%), carbohydrate (38.55±0.00%), crude fat (3.41±0.01%), crude fibre (4.20±0.01%), ash (9.11±0.01%)...